鼠李糖乳杆菌在防御鼠伤寒沙门菌感染中的保护作用

Evaluation of Protective Effect of Lactobacillus rhamnosus against Salmonella typhimurium Infection

为探讨鼠李糖乳杆菌对鼠伤寒沙门菌感染的防御作用,实验拟利用鼠伤寒沙门菌诱导建立小鼠肠炎模型,采用鼠李糖乳杆菌对肠炎模型进行干预。通过检测小鼠的肠道病理变化、器官指数、沙门菌的组织荷菌数、盲肠组织细胞因子的分泌水平及小鼠体重和存活率,明确鼠李糖乳杆菌对沙门菌感染小鼠的保护作用。结果显示,鼠李糖乳杆菌能显著改善沙门菌造成的肠炎损伤,抑制沙门菌向脾、肝脏等器官的侵袭,促进抑炎因子IL-10的分泌,抑制炎性因子NO、IL-12的分泌从而缓解肠道炎症,并能提高感染沙门菌后小鼠的体重增加率和存活率。实验表明,鼠李糖乳杆菌在防御沙门菌感染及改善肠道损伤方面发挥了较好的保护作用。

In order to evaluate the defense effect of Lactobacillus rhamnosus( GG) against Salmonella typhimurium infection,the mouse were treated with Lactobacillus rhamnosus( GG) and then models of enteritis were built up through infecting Salmonella typhimurium. To demonstrate the protective effect,the intestinal pathological score,organ index,bacterial loads in tissue,cytokine expression in cecal tissue,body weight and survival rate were determined in this paper. The results showed that Lactobacillus rhamnosus significantly reduced intestinal inflammation in mice with enteritis,prevented Salmonella typhimurium from invading the intestinal barrier to reside in other organs. It also showed that Lactobacillus rhamnosus increased the secretion of IL-10,decreased the secretion of NO and IL-12,and also promoted the weight gain rate and survival rate,which could be helpful to relieve intestinal inflammation and injury. Therefore,Lactobacillus rhamnosus can display the protective effect against Salmonella infection.