合欢磁牡口服液提取工艺研究

Study on Extraction Technology of Hehuancimu Oral Liquid

为探究不同的提取条件对合欢磁牡口服液质量的影响,以确定最佳的提取工艺。通过不同的提取工艺、浓缩工艺及提取工艺验证试验处理后,检测合欢磁牡口服液中(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷(C33H44O17)的含量。结果显示,煎煮次数C对提取工艺有显著影响(P<0.01);煎煮时间B和加水量A对提取工艺无显著影响(P>0.05);不同的浓缩温度对合欢磁牡口服液含量无显著影响(P>0.05),采用本试验确定的最佳工艺提取的合欢磁牡口服液中C33H44O17的转移率平均值为72.37%,RSD为0.83%。因此,确定的最佳提取生产工艺为8倍量水,提取3次,每次煮沸1 h。

The purpose of this experiment was to investigate the effect of different extraction conditions on the quality of Hehuancimu oral liquid to determine the best extraction technology.After different extraction,concentration and the verification tests of extraction technology,the content of(-)-eugenol-4-O-beta-D-furanin glycosyl-(1→2)-beta-D-pyran glucose(C33H44O17)in Hehuancimu oral liquid was determined.The results showed that the times of decoction(C)had a significant effect on the extraction technology(P<0.01);the time of decoction(B)and the amount of water added(A)had no significant effect on the extraction technology(P>0.05);and there was no significant difference in the effect of different concentration temperature on the content of Hehuancimu oral liquid(P>0.05);the average transfer rate of C33H44O17extracted by the best extraction technology was 72.37%and RSD was 0.83%.Therefore,the optimal extraction production technology was determined as 8 times water,3 times extraction,1 hour boiling each time.