哈尔滨市动物源性金黄色葡萄球菌的分离鉴定及耐药性分析

Isolation,identification and drug resistance analysis of Staphylococcus aureus from animal farms in Harbin

目的为了解哈尔滨市部分地区动物源性金黄色葡萄球菌的耐药情况并进行分析。方法随机选取哈尔滨市南岗区、香坊区、五常市、宾县部分养殖场,采集550份畜禽粪便样品并于Mueller-Hinton高盐平板培养,挑取可疑菌落进行PCR扩增,对确定为金黄色葡萄球菌进行22种临床常用抗菌药物的耐药性试验。结果从550份样品中分离出17株金黄色葡萄球菌,包括10株鸡源菌株、6株猪源菌株和1株牛源菌株。17株临床分离株对22种抗菌药物均有不同程度耐药,对青霉素G的耐药最为严重,耐药率高达76%,对氯霉素耐药的菌株数仅有4株,耐药率为24%。结论研究结果显示分离菌的耐药性严重,多重耐药菌超过菌株总数的一半。本试验结果为研究哈尔滨市动物源性金黄色葡萄球菌耐药机制和耐药传播规律的分析提供了参考,同时为耐药基因在基因水平的研究奠定基础。

Objective To investigate and analyze the resistance of animal derived Staphylococcus aureus in some areas of Harbin.Methods Selecting some farms in Nangang District,Xiangfang District,Wuchang City and Binxian County in Harbin district randomly,gathering 550 samples of livestock manure and culturing on Mueller-HInton high salt plate.The suspicious colonies were amplified by PCR,and then determine the resistance of Staphylococcus aureus to 22 commonly used antibiotics in clinical trials.Results 17 strains of Staphylococcus aureus were isolated from 550 samples,including 10 pip-associated strains,6 chicken-associated strains and 1 cattle-associated strains.The 17 clinical isolates showed different degrees of resistance to 22 kinds of antimicrobial agents,the resistance to penicillin G was the most serious and the rate of drug resistance was 76%.Only 4 strains of bacteria are resistant to chloramphenicol and the resistant rate was 24%.Conclusion The results showed severely resistance of isolates,multi-drug resistant bacteria accounted for 60%of the total number of isolates.The results of this study provide a reference for the study of the mechanism of drug resistance and the transmission of drug resistance in animal derived Staphylococcus aureus in Harbin,as well as lay the foundation for the study of resistance genes at the gene level.