D-82体内外抗肿瘤活性

Antitumor Activity of D-82 in Vivo and in Vitro

目的 探讨二氨基肉豆蔻酸 (D- 82 )体内外抗肿瘤作用。 方法 应用锥虫蓝排染法、MTT法、生长曲线测定法检测 D- 82对多种体外培养肿瘤细胞的细胞毒作用 ;在可耐受剂量下治疗小鼠移植性实体瘤 S1 80、U 1 4 ,观察 D- 82对肿瘤生长的抑制率。 结果 D- 82显著杀伤多种体外培养恶性肿瘤细胞 ,药物作用 4 8h,IC50 2～ 5μg/ m L。 D- 82剂量依赖性地抑制小鼠移植性实体瘤 U 1 4、S1 80体内生长。 结论 D- 82有较强的体内外抗肿瘤活性 。

Objective To investigate antitumor effect of biaminotetradecanoic acid(D|82) in vivo and in vitro. Methods The cytotoxic effects of D|82 on various tumor cell lines cultured in vitro were determined by trypan blue dye exclusion test, MTT method and growth curve method. At a tolerable dose level, D|82 was administrated to treat transplanted solid tumors S180 and U14, tumor weight inhibition was observed. Results D|82 induced significant growth arrested in various malignant tumor cell lines cultured in vitro. IC-{50}of these cells exposed to the drug for 48 hours ranged from 2 to 5 μg/mL. D|82 inhibited in vivo growth of mice implanted solid tumor S180 and U14 in a dose|dependent manner. {Conclusion} D|82 had obvious antitumor activity in vivo and in vitro. D|82 is very hopeful to be a new antitumor drug.