利用RAPD技术筛选家蚕抗核型多角体病分子标记

Studies on RAPD markers for NPV resistance in silkworm(Bombyx mori) using RAPD method

在含有抗核型多角体病毒(NPV)病主效基因的家蚕品种NB和高敏感品种306及其近等基因系BC9中,采用500个RAPD随机引物分别在各个品系的DNA混合物中进行扩增以筛选分子标记,获得与家蚕抗NPV病有关的3个分子标记:OPF 072023、OPJ 131300、OPM 161200 其中OPF 072023标记通过各回交代检测,证明获得的标记真实、可靠 又对该分子标记的片段进行克隆、测序 通过该片段的生物信息学分析。

Two silkworm strains, namely, NB which is highly resistant to NPV, and 306 which is highly susceptible to NPV, and their near-isogenic line BC_(9),were used in the study. 500 RAPD random primers were used to screen molecular markers in DNA mixtures of these strains. Three molecular markers, OPF-07, OPX-06 and OPX-18 were found linked to major gene resistant to NPV. One molecular marker, OPF-07_(2023), was validated in some backcrossing generations. This special band appearing only in the resistant strains was cloned, sequenced and analyzed. With bioinformatic analysis, a retrotransposon gene of the Z chromosome in silkworm was found unexpectedly.