纯镁超声微弧氧化载人乳铁蛋白复合膜层植入体的生物相容性研究

Biocompatibility of pure magnesium implants processed by ultrasonic microarc oxidation and coupled with human lactoferrin

目的通过对纯镁表面超声微弧氧化(UMAO),硅烷偶联载人乳铁蛋白(HLF),期望提高医用Mg在动物体内的早期骨整合能力及调控其降解速率。方法纯镁超声微弧氧化(UMAO)为对照A组,UMAO-硅烷为实验B组,UMAO-硅烷-0. 1μg/L的人乳铁蛋白为实验C组,分别将不同处理的36个镁骨钉随机植入家兔单侧下颌骨中,2周、4周、8周后分期处死家兔,截取载钉下颌骨,进行口腔数字化锥形束CT(CBCT)观察骨结合及植体钉表面吸收情况,激光共聚焦、扫描电镜、能谱分析以及甲苯胺蓝染色观察细胞形态、界面元素分布及成骨性。结果扫描电镜观察经UMAO-硅烷-人乳铁蛋白复合膜层处理后C组骨钉表面空隙逐渐缩小实现封孔,CBCT观察第8周植体钉表面附着新生骨组织能力及耐降解能力均为C组>B组>A组,甲苯胺蓝染色显示镁钉周围新生骨细胞及骨组织能力C组>B组>A组。结论纯镁骨钉经超声微弧氧化硅烷偶联载人乳铁蛋白后,具有良好的促进骨结合和早期骨生长的能力,可减缓在动物体内降解速率。

Objectives By ultrasonic micro-arc oxidation(UMAO)and silane coupling with human lactoferrin(HLF)on the surface of pure magnesium,it is to improve the early osseointegration ability of medical Mg in animals and regulate its degradation rate.Methods 36 differently treated magnesium bone nails were implanted into the unilateral mandible of rabbits.Pure Magnesium Ultrasound Microarc Oxidation(UMAO)was controlled group A,UMAO-Silane was experimental group B,UMAO-silane-0.1μg/L human lactoferrin was experimental group C.Rabbits were sacrificed 2 weeks,4 weeks and 8 weeks after implanting.The mandibles loaded with nails was intercepted and the bone bonding and surface absorption of the implant nails were observed by oral digital cone-beam computed tomography(CBCT).Laser confocal scanning electron microscopy(LC),energy dispersive spectroscopy(EDS),and toluidine blue staining were used to observe cell morphology,interface element distribution and osteogenesis.Results Scanning electron microscopy(SEM)showed that the surface voids of bone nails in group C were gradually narrowed and sealed after treatment with UMAO-silane-human lactoferrin composite membrane.CBCT showed that the ability of attaching new bone tissue to the surface of implant nails is group C>group B>group A at week 8.Toluidine blue staining showed that the ability of new bone cells and bone tissue around the nails is group C>group B>group A>group B>group A.Conclusions Pure magnesium bone nails coupled with human lactoferrin by ultrasonic micro-arc oxidation silane have good ability to promote bone binding and early bone growth,and can slow down the degradation rate in vivo.