小麦新品种川农16的分子鉴定

Molecular Identification of New Wheat Cultivar (Chuannong) 16

为有效利用基因资源,应用RAPD、STS和SSR等3种分子标记对小麦新品种川农16及其亲本和对照品种进行了分析鉴定。结果表明,供试材料在DNA水平上存在多态性。3种标记均能揭示川农16与对照品种在DNA水平上的遗传差异。川农16与双亲在相同位点的同源性也有差异,电泳带型表现出同父、同母、综合和不同于亲本的新带型等多种类型。其中,RAPD分析中共有22个引物(32.8%)和68条(42.8%)带纹、STS分析中有6个引物(50%)和21个引物-酶组合(17.5%)、而SSR分析中有11个位点(32.4%)能揭示材料间的差异。与RAPD和STS标记相比,SSR标记结合聚丙烯酰胺凝胶电泳和银染技术更适合于绘制小麦指纹图谱。

In order to utilize gene resource efficiently, the genetic variations among new wheat cultivar (Chuannong) 16,its parents (i.e. Chuanyu 12 and 87-429) and other cultivars (i.e. Chuanmai 28 and Mianyang 26) were analyzed and identified by RAPD, STS and SSR markers. The results indicated that the genetic (differences) among (Chuannong) 16 and its parents were detected and all materials could be distinguished by the three kinds molecular (markers). 68 polymorphic bands were observed in 22 of 67 random primers (32.8%) in RAPD analysis, Genetic (differences) were revealed in 6 of 12 primer-sets (50%) and 21 of 120 (primer-enzyme) combinations (17.5%) in STS analysis, and in 11 of 34 SSR primer-sets (32.4%) in SSR analysis. In this study, the results suggested that SSR (markers) combining with (polyacrylamide-)(gel-electrophoresis) were superior to RAPD and STS markers in wheat cultivar identifying and fingerprinting.