利用酵母双杂交系统筛选与草莓镶脉病毒移动蛋白P1互作的寄主因子

Screening of the host factors of woodland strawberry interacting with P1 of Strawberry vein banding virus by yeast two-hybrid system

构建感染草莓镶脉病毒(SVBV)森林草莓的酵母c DNA文库,利用酵母双杂交系统,筛选出与SVBV P1蛋白互作的15种寄主因子。生物信息学分析发现,这15种寄主因子参与茉莉酸途径、泛素化、光合作用、抗病抗逆、蛋白修饰、蛋白运输和氧化还原等多种生物过程。另外,这些寄主因子还具有其他分子功能,包括氧化还原酶活性、蛋白二硫化物异构酶活性和金属离子结合活性等。本研究初步探讨了P1与寄主因子的互作机理,为揭示SVBV侵染森林草莓以及SVBV在寄主中扩展的分子机制提供理论依据。

A yeast cDNA library of woodland strawberry infected with Strawberry vein banding virus ( SVBV) was constructed,and 15 SVBV P1-interacting host factors were screened through a yeast two-hybrid system. Bioinformatics analysis showed that these genes were involved in various biological processes such as jasmonic acid pathway,ubiquitination,photosynthesis,defense response,protein modification,protein transportation and oxidation-reduction process. In addition,these factors also possessed other molecular functions,including oxidoreductase activity,protein disulfide isomerase activity and metal ion binding activity,etc. This study preliminarily discussed the interaction mechanism between P1 and the host factors,providing a theoretical basis for revealing the molecular mechanism of SVBV infection and transport in woodland strawberry.