来源于湖北省梨轮纹病菌中真菌病毒多样性检测及其分析

Detection and analysis of the double-stranded RNAs diversity from Botryosphaeria dothidea strains in Hubei province

一些真菌病毒严重影响寄主生长、产孢量、致病力。本研究对来源于湖北梨轮纹病菌携带真菌病毒情况进行分析,旨在挖掘具有生防潜能的真菌病毒资源。随机采集湖北省梨树发病枝干,分离、鉴定获得13个梨轮纹病菌株,其生长速率、致病性存在差异。提取dsRNA检测,发现12个菌株均携带不同大小的dsRNA片段。观察其dsRNA带型,HBWH-14 dsRNA含有1～7 kb 9条带,其他菌株携带的dsRNA条带位置均位于HBWH-14所含dsRNA带型中的某1条或多条对应的条带位置。RT-PCR检测发现部分菌株携带产黄青霉病毒BdCV1、双分体病毒BdPV1、单分体病毒BdTV1以及未分类的BdRV1。本研究未发现梨轮纹菌株所含病毒种类与致病力存在直接的相关性。HBWH-14原生质体脱毒获得后代100%携带的dsRNAs稳定遗传。

Some mycoviruses have effect on growth rate,sporulation ability,pathogenicity biological characteristics of fungi host. Aims at detection of dsRNA mycoviruses infecting Botryosphaeria dothidea strains collected from Hubei province,which are to be explored as bio-control resources. The obtained results are the followings. 13 strains were isolated and cultured from pear branches with symptoms of ring spot disease to some extent in Wuhan city of Hubei province. The growth rate showed differences among 13 B. dothidiea strains,which exhibit different virulence. The patterns of dsRNAs were detected. The results show that 12 B. dothidiea strains have different sizes of molecular weight. The dsRNA profiles analysis indicate that the dsRNAs from HBWH-14 strain contain 9 bands with a molecular weight range of 1-7 kb,meanwhile the other strains exhibit one or more than dsRNA bands in accordance with that of HBWH-14 strain. In total,single or mixed infection with BdCV1,Bd- PV1,BdRV1 and a new member of Totiviridae ( designated as BdTV1) are positive for the above one or more strains by RT-PCR detection;There was no correlation between mycoviruses and pathogenicity for 13 strains. dsRNAs from HBWH-14 regenerated strains are 100% positive by protoplast isolation.