MfSre1基因参与调控桃褐腐病菌对外源渗透的胁迫和对DCFs杀菌剂敏感性

MfSre1 gene is associated with the osmotic tolerance and DCFs resistance in Monilinia fructicola

美澳型核果链核盘菌(Monilinia fructicola)引起的褐腐病是严重威胁桃生产的重要真菌性病害。为揭示该病原菌的抗药性机制和发掘新的药剂靶标,本研究利用同源重组分割标记法对MfSre1基因进行了敲除,并研究了该基因的生物学功能。与亲本甾醇14-α-脱甲基化酶抑制剂(DMI)抗性菌株Bmpc7相比,敲除转化子的菌落形态、菌丝生长速率、产孢能力和致病力没有显著变化(P>0.5),对DMIs杀菌剂的敏感性、过氧化氢和高浓度甘油渗透胁迫也没有显著性区别。但对金属离子、糖类、细胞壁/细胞膜破坏剂的敏感性明显降低,对二甲酰亚胺类杀菌剂(DCFs)的抗性水平显著上升。这些结果表明,MfSre1参与调控对部分外源渗透的胁迫和对DCFs杀菌剂的敏感性。

Brown rot caused by Monilinia fructicola is an important fungal disease in peach production. In order to demonstrate the resistance mechanism and explore the molecular target of fungicide,MfSre1 was knocked out by using the Split Marker method and PEG-mediated protoplast transformation. Compared with the parental demethylase inhibitors ( DMI) fungicide-resistant strain Bmpc7,there was no significant difference in the mycelial growth,sporulation and pathogenicity of knockout transformants ( P>0.5) . Also,there was no significant difference in the sensitivity to DMIs fungicides,hydrogen peroxide,and glycerol osmotic stress. However,the sensitivities to metal ions,saccharides compounds,cell wall and cell membrane damage agent were significantly decreased, and the resistance to dicarboximide fungicides ( DCFs) increased significantly. These results indicate that MfSre1 is involved in regulating the exogenous stress and DCFs fungicide resistance.