摘要
大青(Clerodendrum cyrtophyllum Turcz),别名路边青、淡婆婆、臭叶树,为马鞭草科(Verbena officinalis Linn)大青属(Clerodendrum)野生落叶灌木,常生长在山地林下、平原、丘陵和溪谷旁,我国江苏、安徽、河北、河南、浙江等地为主产区。
Clerodendrum cyrtophyllum leaf samples showing golden mosaic symptom were collected from Hangzhou and Huzhou city of Zhejiang province,and used for sRNA library construction and deep sequencing.After assembly of total sRNAs,only Clerodendrum golden mosaic China virus(ClGMCNV)was identified in the samples.The complete genomic fragments of ClGMCNV were obtained by PCR,and confirmed by sanger sequencing.The results showed that the full genomic sequence of DNA-A were 2 776 bp in both isolates,and shared 99%nucleotide similarity with the DNA-A of ClGMCNV isolate YX1(GenBank accession number FN396962.1).The full length of DNA-B were 2 729 bp and 2 732 bp in HZ and Hu Z isolates respectively,and both shared 98%nucleotide similarity with the DNA-B of ClGMCNV isolate YX1(GenBank accession number FN396963.1).Phylogenetic analysis with complete genomic sequences indicated that the three isolate(YX1,HZ,Hu Z)were clustered into one group,but the relationship of Hu Z isolate was closer to YX1.This is the first report of ClGMCNV from C.cyrtophyllum in Zhejiang province.
作者
苏秀
陈莎
周湘
龙奎
刘颖
郭恺
SU Xiu;CHEN Sha;ZHOU Xiang;LONG Kui;LIU Ying;GUO Kai(National Joint Local Engineering Laboratory of Biopesticide High-efficient Preparation,School of Forestry and Biotechnology,Zhejiang Agriculture and Forestry University,Lin'an 311300,China;College of Sciences and Chemistry,Hunan University of Technology,Zhuzhou 412007,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2019年第3期424-427,共4页
Acta Phytopathologica Sinica
基金
浙江省自然科学基金项目(LQ17C160003)
