甲状腺乳头状癌中miR-155、SOCS1的表达及临床意义

Expression and clinical significance of mir-155 and SOCS1 in thyroid papillary carcinoma

目的探讨甲状腺乳头状癌(PTC)组织中miR-155及SOCS1基因的表达情况,分析两者表达与PTC临床病理的相关性。方法在60例PTC组织、20例正常甲状腺组织中采用实时荧光定量RT-PCR法分别检查miR-155、SOCS1基因mRNA的表达水平;分析PTC与正常甲状腺组织中miR-155、SOCS1的表达差异及其与PTC临床病理的相关性。结果 PTC中miR-155表达量高于正常甲状腺组织,而PTC中SOCS1基因表达量低于正常甲状腺组织,两者差异均有统计学意义(P<0.05);PTC中miR-155、SOCS1基因的表达水平与肿瘤TNM分期、病理分级及淋巴结转移均相关(P<0.05),而与年龄、性别及肿瘤大小等因素无关(P>0.05)。在PTC中miR-155、SOCS1基因的表达呈负相关(r=-0.564,P<0.05)。结论 PTC中miR-155、SOCS1基因表达异常,提示miR-155的表达上调可能通过调控SOCS1的表达参与了PTC发生发展,miR-155、SOCS1基因两者有可能成为PTC治疗新的靶点。

Objective To investigate the expression of miR-155 and suppressor of cytokine signaling-1(SOCS1)in thyroid papillary carcinoma(PTC),and to analyze the correlation between the two expressions of PTC with clinic pathology.Methods The expressions of miR-155 and SOCS1 mRNA were examined by real-time fluorescence quantitative RT-PCR method in 60 cases of PTC tissues and 20 cases of normal thyroid tissues.The expressions of miR-155 and SOCS1 between PTC and normal thyroid tissues were analyzed,and its correlation with PTC clinical pathology was also analyzed.Results The expression of miR-155 in PTC was higher than that in normal thyroid tissue,while lower than that in normal thyroid tissue,and both of the differences were statistically significant(P<0.05).The expression of miR-155 and SOCS1 in PTC pathological grading,TNM stages and lymph node metastasis in patients were all related(P<0.05),while it had no correlation with the factors like gender,age and tumor size(P>0.05).The expressions of miR-155 and SOCS1 in PTC were negatively correlated(r=-0.564,P<0.05).Conclusion Abnormal expressions of miR-155 and SOCS1 in PTC were observed,and the expression increase of miR-155 may participate in the development of PTC through regulating the expression of SOCS1.Both miR-155 and SOCS1 could be become the new treatment targets of PTC.