糜子叶片内源激素及保护系酶对黑穗病菌胁迫的响应

Study on responses of leaf protective enzyme and endogenous hormone to smut fungus stress in broomcorn millet

为了探究不同品种糜子叶片的内源激素及保护系酶对黑穗病菌胁迫的响应,本实验以4个不同抗性水平的糜子品种为材料,测定了各材料各时期顶三叶的内源激素含量以及保护系酶的活性。结果表明,感染黑穗病菌后,各品种糜子叶片的生长素和赤霉素含量降低,细胞分裂素、脱落酸、水杨酸、茉莉酸和丙二醛含量增加,超氧化物歧化酶和过氧化物酶活性增加。大部分时期,抗病品种糜子叶片的细胞分裂素、水杨酸和JA含量均显著高于感病品种,而生长素、赤霉素和脱落酸含量在抗、感品种间无显著差异。抗病品种糜子叶片的过氧化物酶活性显著高于感病品种,而丙二醛含量显著低于感病品种。感病早期,抗病品种叶片超氧化物歧化酶活性显著高于感病品种,但在后期则显著低于感病品种。

In order to explore the response of endogenous hormones and protective enzymes to the stress of head smut in the leaves of different varieties of broomcorn millet,four broomcorn millet cultivars with different resistance levels were used as materials.The endogenous hormones contents and protective enzymes activities of the top three leaves were determined at various stages.The results showed that,after infecting with smut,the contents of auxin and gibberellin in the leaves were decreased,the contents of cytokinin,abscisic acid,salicylic acid,jasmonic acid,and MDA were increased,and the activities of SOD and POD were increased.In most periods,the contents of cytokinin,salicylic acid and jasmonic acid in resistant cultivars were significantly higher than those in susceptible cultivars,while there were no significant differences in the contents of auxin,gibberellin and abscisic acid between resistant and susceptible cultivars.The POD activities of resistant varieties were significantly higher than those of susceptible varieties,while the MDA contents were significantly lower than those of susceptible varieties.During the early stage of smut infection,the SOD activities in resistant varieties were significantly higher than those of susceptible varieties,but in the late infection stage,they were significantly lower than those of susceptible varieties.