月季生长素结合蛋白基因RcABP19的克隆及表达特征分析

Cloning and expression profile analysis of auxin binding protein gene RcABP19 in Rosa chinensis

本研究以月季品种‘月月粉’和‘绿萼’为试验材料,对生长素结合蛋白基因RcABP19进行克隆和表达分析,并通过转化拟南芥和在月季中进行病毒诱导基因沉默(VIGS)等途径,对其在花器官发育中的作用进行了研究。结果表明:RcABP19属于Cupin超家族成员。喷施外源IAA、IBA和6-BA后, RcABP19的表达量显著升高,表明RcABP19受到其诱导。此外, RcABP19的表达响应昼夜节律, RcABP19在白天随着光照时间的增加而积累,在傍晚时达到峰值,之后显著降低。RcABP19基因在2个月季品种的花器官发育中的表达趋势存在显著差异,且均在花器官中高表达。过表达RcABP19拟南芥和在月季中进行RcABP19病毒诱导基因沉默(VIGS)均未观察到明显的花器官表型变化,但其相应的花器官发育相关基因(AP1、AP3和PI基因)的表达变化显著。本研究初步证明,月季RcABP19基因的表达响应植物生长调节物质信号及光周期节律,且可能参与花器官发育调控。

The Chinese old rose cultivars Rosa chinensis‘Old Blush’and‘Viridiflora’(known as green rose)were used in our study as plant materials,and then the cloning and gene expression analysis of RcABP19 gene were performed and compared between these two cultivars.Arabidopsis transformation and VIGS gene silencing in rose were applied for further characterization of RcABP19 functions in flower development.The results showed that RcABP19 gene was one of the Cupin super family members,and which was significantly induced by IAA,IBA and 6-BA treatments.In addition,the expression of RcABP19 also responsed to circadian rhythm during a day,RcABP19 accumulated in the daytime with the increase of illumination time,and reached the peak in the evening before getting dark,and then decreased significantly.The expression of RcABP19 significantly varied in floral organ development of two cultivars,and all of them were highly expressed in floral organs.Furthermore,although there were no visible phenotypes differences observed in over-expressed transgenic Arabidopsis lines and VIGS gene silencing rose lines,the expression of flower development related genes,especially AP1,AP3 and PI,changed significantly.Our results suggested that the expression of RcABP19 gene responsed to plant growth regulators and circadian rhythm in rose,and might be involved in the regulation of plant flower development.