宁夏水稻代表性种质及后代抗稻瘟病基因型分析

Analysis of Blast Resistance Genes in Typical Japonica Rice Germplasm and Progeny in Ningxia

以96份宁夏水稻代表性种质资源和47份杂交后代为试验材料,采用宁夏稻瘟病菌生理小种人工接种和自然诱发相结合的方法对其进行稻瘟病抗性的大田鉴定和病圃鉴定,评价了每个材料的稻瘟病抗性;同时采用8个主效抗稻瘟病基因的功能标记对上述材料进行抗病基因的分子检测,得到了28个不同的抗稻瘟病基因型,将不同基因型的稻瘟病抗性评价进行了差异显著性分析。结果表明,同时含有Pita、Pib、Pikm、Pikh、Pi9和Pi5共6个抗病基因组成的基因型的水稻材料对稻瘟病的抗性极显著高于其他基因型的抗性。Pita基因单独与Pikm、Pikh、Pi2、Pi5基因聚合时均表现出正向聚合效应,其中Pita与Pikh基因的聚合效应最强。当Pib与Pita+Pikh聚合时,表现出负向聚合效应。这为宁夏地区通过基因聚合方法选育广谱持久抗稻瘟病水稻新品种提供了重要的参考依据。

In this study,ninety-six Typical japonica rice germplasm resources and these 47 hybrid progeny in Ningxia province were tested for rice blast resistance in the fields,by using artificial inoculation and natural infection with physiological races of rice blast pathogen.Moreover,eight functional markers,which associated to the respective rice blast resistance genes,were used for marker-assisted detection of the resistance genes in mentioned materials as well as additional 28 different Genotypes.As observed,the resistance of genotypes containing Pita,Pib,Pikm,Pikh,P9 and Pi5 represented significantly high level of resistance than that of other genotypes.The Pita gene,pyramiding with either of Pikm,Pikh,Pi2 and Pi5 genes,showed increased resistance to rice blast,and notably,stacking of Pita and Pikh genes showed highest resistance.However,Pib and Pita+Pikh in a genotype showed reduced rice blast resistance.Thus,this work provides an important reference in breeding for new rice varieties with broad-spectrum resistance,by pyramiding of rice blast resistance genes in Ningxia region.