摘要
探讨SSR分子标记用于荞麦种间F1杂种的真假性鉴定的可行性,为今后荞麦杂交育种研究及高产优质新品种选育提供一定的指导。利用235对SSR引物对小米荞、大苦1号、红心金荞、大野荞、巨荞5个亲本进行PCR扩增,以获得多态性较高的引物,在此基础上对大苦1号×红心金荞、巨荞×红心金荞、小米荞×红心金荞、巨荞×小米荞、巨荞×小米荞B、巨荞×大野荞6个杂交组合杂种进行SSR分子标记分析,同时对真杂种形态表现进行观察记录并计算其杂种优势。结果获得2对多态性较高引物(SSR2159/58和SSR6790/89),鉴定出3个真杂种:大苦1号×红心金荞、小米荞×红心金荞、巨荞×红心金荞。本研究结果说明SSR分子标记可以用于荞麦种间杂种鉴定,真杂种大苦1号×红心金荞表现出超亲优势且正常可育,可用于新品种选育。
This study attempted to search SSR markers for validating the authenticity of F1 hybrids between buckwheat species,in order to provide some clues for the future research of crossing breeding and the development of new varieties with high yield and good quality.By employment of 235 pairs of SSR primers,we performed PCR amplification using 5 parental accessions,including Fagopyrum tataricum(L.)Gaertn.(Xiaomiqiao),F.tataricum(L.)Gaertn.(Daku No.1),F.cymosum(Trevir.)Meisn.,F.megaspartanium Q. F. Chen,F.giganteum Krotov to find the polymorphic markers.On this basis,primers with higher polymorphism were obtained,and SSR molecular marker analysis of 6 hybrids,including F.tataricum(Daku No.1)×F.cymosum,F.giganteum×F.cymosum,F.tataricum(Xiaomiqiao)×F.cymosum,F.giganteum×F.tataricum,F.giganteum×F.tataricum,F.giganteum×F.megaspartanium.At the same time,the morphological characteristics of hybrids were observed and recorded,and their heterosis was calculated.The results showed that there are 2 pairs of polymorphic primers(SSR2159/58 and SSR6790/89)identified,and 3 true hybrids were identified,including F.tataricum(Daku No.1)×F.cymosum,F.tataricum(Xiaomiqiao)×F.cymosum,F.giganteum×F.cymosum(Trevir.)Meisn..Thus,SSR markers can be used for identifying interspecific hybrids of buckwheat,hybrid F.tataricum(Daku No.1)×F.cymosum showed superparental superiority and normal fertility,and could be used for developing varieties.
作者
杨丽娟
饶庆琳
李洪有
王艳
冉盼
崔娅松
陈庆富
YANG Li-juan;RAO Qing-lin;LI Hong-you;WANG Yan;RAN Pan;CUI Ya-song;CHEN Qing-fu(Research Center of Buckwheat Industry Technology,Guizhou Normal University,Guiyang 550001)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第2期387-395,共9页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31471562
31860408)
国家燕麦荞麦现代农业产业技术体系专项资金(CARS-07-A5)
贵州省高层次创新型人才培养对象十百千计划(2014GZ97588)
贵州省荞麦工程技术研究中心(黔科合农G字【2015】4003号)
贵州省农业科技支撑计划(黔科合支撑【2017】2505
黔科合支撑[2018]2320)~~
关键词
荞麦
种间杂种
SSR鉴定
形态表现
buckwheat(Fagopyrum spp.)
hybrid F1
SSR identification
morphdogy