SSR标记在辣椒DUS测试中的应用研究

Study on the Application of SSR Markers in Pepper(Capsicum annuum L.)DUS Testing

为了评价SSR分子标记在辣椒(Capsicum annuum L.)品种特异性、一致性、稳定性(DUS)测试中的应用潜力,采用聚丙烯酰胺凝胶电泳技术和毛细管电泳技术,对辣椒SSR引物进行筛选,采用入选的30对核心引物,构建130份不同来源品种的指纹图谱。30对引物共检测到等位基因163个,每对引物等位基因变幅为2～15,平均等位基因数为5.43,多态信息量(PIC)的变异范围为0.17～0.76,平均为0.43。聚类分析表明,该套引物可以将所有参试品种区分开来,遗传基础相近的品种优先聚在一起。130个品种的遗传距离为0.01～0.91。有20对品种遗传距离小于0.10,其中3对品种有1个位点的差异,采用差异位点数判断品种特异性更适合目前辣椒品种现状。采用10对引物对品种0313963-4进行了一致性检测,综合一致性比率R值为96.5%,低于DUS测试中对表型性状一致性的要求。因此SSR标记直接用于辣椒DUS判定还需进一步研究,但是可用于近似品种筛选。

To evaluate the application of SSR markers in pepper DUS testing,here we employed thirty pairs of SSR primers in fingerprinting analysis of 130 varieties.A total of 163 polymorphic alleles were detected by30 primer pairs,which ranged from 2 to 15 with a mean value of 5.43.The polymorphism information content(PIC)ranged from 0.17 to 0.76,with the average of 0.43.These genotypes can be clarified using the polymorphic information.These pepper cultivars with close genetic background were clustered.The genetic distance of the 130 cultivars ranged from 0.01 to 0.91.There were 20 pairs of cultivars whose genetic distance was less than 0.10,among which 3 pairs had one locus difference.It was more suitable to judge the distinctness of pepper varieties by using the number of difference locus.Ten pairs of primers were used to detect the uniformity of the variety0313963-4 and the comprehensive uniformity ratio R value was 96.5,which was lower than the requirement of phenotypic trait in DUS test.Therefore,SSR markers directly used in pepper DUS determination still need further study,but it can be used in the selection of similar varieties.