miR-935的表达与进展期胃癌患者含铂方案化疗疗效以及预后的相关性研究

Relationship of expression of miR-935 with efficacy and prognosis of platinum chemotherapy for advanced gastric cancer

[目的]探讨进展期胃癌患者肿瘤组织中miR-935的表达与患者临床病理特征、含铂方案化疗疗效以及预后的关系。[方法]选取2012年1月～2013年10月在我院胃肠肿瘤外科行根治术的进展期胃癌患者367例,采用实时定量聚合酶链式反应(qRT-PCR)检测进展期胃癌患者肿瘤组织和配对的癌旁组织中miR-935的表达量。采用χ~2检验分析与患者病理特征的关系。记录患者总生存时间(OS),并采用单因素和多因素Cox风险比例模型分析影响患者OS的因素。[结果]肿瘤组织中miR-935的表达量明显高于癌旁组织(P<0.05),其高表达率为63.76%。miR-935表达量与肿瘤分化、N分期、TNM分期存在明显相关性(P<0.05)。经过4周期含铂方案的化疗,239例(65.12%)患者化疗有效,128例(34.88%)患者化疗无效。有效组患者肿瘤组织miR-935表达量明显低于无效组患者(P<0.05)。经Kaplan-Meier曲线分析,miR-935低表达组患者PFS较高表达组患者更长(log-rank:χ~2=5.961,P<0.05)。经单因素和多因素Cox风险比例模型分析,淋巴结转移、TNM分期、化疗疗效以及肿瘤组织miR-935表达量是PFS的独立危险因素(P<0.05)。[结论]进展期胃癌患者癌组织中miR-935的高表达与含铂方案近期化疗疗效和预后较差有关。

[Objective]To investigate the relationship between the expression of miR-935 and clinicopathological features,curative effect of platinum regimen and prognosis in patients with advanced gastric cancer.[Methods]A total of 367 patients with advanced gastric cancer who underwent radical radical surgery were enrolled in the Department of Gastrointestinal Surgery,First Affiliated Hospital of Hebei North University from January 2012 to October 2013.The levels of miR-935 in paired cancer tissue and its adjacent cancer tissue in advanced gastric cancer were detected by quantitative real time polymerase chain reaction(qRT-PCR).And relationship between expression of miR-935 and clinical features was analyzed byχ2 test.The overall survivals(OS)of patients were recorded.The influence factors of prognosis were analyzed bu univariate and multivariate Cox proportional harzard regression model.[Results]Relative miR-935 level in cancer tissues was higher than that in paired adjacent cancer tissues(P<0.05).And the high expression rate of miR-935 was 63.76%,which was related with tumor differentiation,N stage and TNM stage(P<0.05).After 4 cycles of platinum chemotherapy,there were 239 patients(65.12%)with effective chemotherapy and 128 patients(34.88%)with non-effective chemotherapy.Relative miR-935 level in cancer tissues of patients with effective chemotherapy was lower than that of patients with non-effective chemotherapy(P<0.05).The OS of patients with low miR-935 levels was longer than that of patients with high miR-935 levels(log-rank:χ2=5.961,P<0.05).The lymph metastasis and TNM stage,chemotherapy efficacy and miR-935 level in cancer tissues were the independent risk factors of PFS(P<0.05).[Conclusion]The high expression of miR-935 in cancer tissues of patients with advanced gastric cancer is related to the short-term chemotherapy efficacy and poor prognosis of platinum-containing regimen.