摘要
AIM: To investigate the mechanisms of chloride intracellular channel 1 (CLIC1) in the metastasis of colon cancer under hypoxia-reoxygenation (H-R) conditions.
AIM:To investigate the mechanisms of chloride intracellular channel 1(CLIC1)in the metastasis of colon cancer under hypoxia-reoxygenation(H-R)conditions.METHODS:Fluorescent probes were used to detect reactive oxygen species(ROS)in LOVO cells.Wound healing assay and transwell assay were performed to examine the migration and invasion of LOVO cells.Expression of CLIC1 mRNA and protein,p-ERK,MMP-2and MMP-9 proteins was analyzed by reverse transcription-polymerase chain reaction and Western blot.METHODS:H-R treatment increased the intracellular ROS level in LOVO cells.The mRNA and protein expression of CLIC1 was elevated under H-R conditions.Functional inhibition of CLIC1 markedly decreased the H-R-enhanced ROS generation,cell migration,invasion and phosphorylation of ERK in treated LOVO cells.Additionally,the expression of MMP-2 and MMP-9 could be regulated by CLIC1-mediated ROS/ERK pathway.CONCLUSION:Our results suggest that CLIC1 protein is involved in the metastasis of colon cancer LOVO cells via regulating the ROS/ERK pathway in the H-R process.
基金
Supported by The "Eleventh Five-year Plan" for Medical Sci-ence Development of PLA,No.06MB243
the National Natural Science Foundation of China,No.81101101 and No.51273165
the Key Project of Chinese Ministry of Education,No.212149
the Projects of Sichuan Province,No.2010SZ0294,No.2011JQ0032 and No.12ZB038
