Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation

AIM To investigate the role of heat shock protein(HSP)-glycoprotein(gp)96 in dendritic cells(DCs)and lymphocytes induction in gastric cancer(GC). METHODS Human GC cell lines KATOIII,MKN-28 and SGC-7901were infected with adenovirus gp96 at a multiplicity of infection of 100.gp96-GC antigen peptide complexes were purified.MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)assay,lactate dehydrogenase(LDH)release assay and enzymelinked immunosorbent assay were used to determine allo-reactive T cell stimulation,natural killer(NK)cell activity and expression of cytokines(such as interleukin(IL)-10,IL-12,interferon(IFN)-γand tumor necrosis factor(TNF)-α),respectively.Effect of cytotoxic T lymphocyte(CTL)on DCs incubated with HSP-gp96was also evaluated by LDH release.All assays were performed in triplicate and the average values were reported.Comparison between groups was conducted using Student’s t test.RESULTS T cells incubated with HSP-gp96 exhibited a marked increase in proliferation in a dose-dependent manner(P<0.05).NK cell activity after gp96-GC peptide complex treatment was significantly higher than that after antigen peptide treatment(P<0.05).The activity of CTLs incubated with DCs from three GC cells lines was obviously higher than that stimulated by GC antigen at ratios of 50:1,25:1,10:1,and 5:1(P<0.05).Furthermore,the secretion of TNF-α,IL-10,IL-12(P70)and IFN-γmarkedly increased after incubation with HSP-gp96(P<0.05).CONCLUSION HSP-gp96 promotes T cell response,enhances DC antigen presentation and induces cytokine secretion,as well.HSP-gp96 has potential as immunotherapy for elimination of residual GC cells.

AIM To investigate the role of heat shock protein (HSP)glycoprotein (gp) 96 in dendritic cells (DCs) and lymphocytes induction in gastric cancer (GC). METHODS Human GC cell lines KATOIII, MKN-28 and SGC-7901 were infected with adenovirus gp96 at a multiplicity of infection of 100. gp96-GC antigen peptide complexes were purified. MTT (3-(4,5-dimethylthiazol-2-yl)2,5- diphenyltetrazolium bromide) assay, lactate dehydrogenase (LDH) release assay and enzyme-linked immunosorbent assay were used to determine allo-reactive T cell stimulation, natural killer (NK) cell activity and expression of cytokines (such as interleukin (IL)-10, IL-12, interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha), respectively. Effect of cytotoxic T lymphocyte (CTL) on DCs incubated with HSP-gp96 was also evaluated by LDH release. All assays were performed in triplicate and the average values were reported. Comparison between groups was conducted using Student's t test. RESULTS T cells incubated with HSP-gp96 exhibited a marked increase in proliferation in a dose-dependent manner (P < 0.05). NK cell activity after gp96-GC peptide complex treatment was significantly higher than that after antigen peptide treatment (P < 0.05). The activity of CTLs incubated with DCs from three GC cells lines was obviously higher than that stimulated by GC antigen at ratios of 50: 1, 25: 1, 10: 1, and 5: 1 (P < 0.05). Furthermore, the secretion of TNF-alpha, IL-10, IL-12 (P70) and IFN-alpha markedly increased after incubation with HSP-gp96 (P < 0.05). CONCLUSION HSP-gp96 promotes T cell response, enhances DC antigen presentation and induces cytokine secretion, as well. HSP-gp96 has potential as immunotherapy for elimination of residual GC cells.