摘要
目的探讨GOLPH3在食管癌转移中的作用。方法通过划痕实验、Transwell侵袭实验和3D培养等方法,研究GOLPH3对KYSE-140细胞迁移和侵袭能力的影响。用q RT-PCR法检测CYR61,CD44与Snail这三个上皮-间质转化(epithelial-mesenchymal transition,EMT)相关分子与GOLPH3的关系,初步探索GOLPH3对食管鳞癌细胞转移能力的影响。结果 GOLPH3对食管鳞癌细胞的迁移和侵袭有促进作用,且CYR61,CD44与Snail在食管鳞癌细胞中的表达水平与GOLPH3的表达呈正相关。结论 GOLPH3高表达可促进食管鳞癌细胞的上皮-间质转化,从而促进食管癌的转移和进展,发挥癌基因作用。
Objective To investigate the role of GOLPH3 in esophageal squamous cell carcinoma(ESCC).Methods Wound healing assays,transwell invasion assays and 3D culture were carried out to analyze the cell migration and invasion ability of GOLPH3 overexpression and knockdown KYSE-140 cells.The relationship between GOLPH3 expression and CYR61,CD44 and Snail m RNA expression was further examined through q RT-PCR,to identify the mechanisms involved.Results GOLPH3-promoted ESCC cell migration and invasion.CYR61,CD44 and Snail m RNA expression levels were correlated with GOLPH3 protein expression level.Conclusions GOLPH3 overexpression promotes ESCC metastasis through epithelial-mesenchymal transition(EMT),and plays an oncogenesis role in ESCC.
出处
《中国胸心血管外科临床杂志》
CAS
CSCD
2015年第7期672-677,共6页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
广东省自然科学基金项目资助(S2013010011548)~~
关键词
食管癌
GOLPH3
上皮-间质转化
Esophageal carcinoma
GOLPH3
Epithelial-mesenchymal transition
