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中药板蓝根提取液致细胞增殖的研究

Evaluating effects of Radix Isatidis extraction on cell proliferation
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摘要 目的:评价板蓝根提取液对细胞相对增殖活力的影响及相关机制的研究。方法:通过MTT及CCK-8法考察板蓝根提取液对14种不同来源细胞相对增殖活力的影响;通过Western Blotting法考察板蓝根提取液对MDCK增殖通路中p-Erk的活化程度;通过Erk抑制剂作用之后考察板蓝根提取液对MDCK增殖的改变。结果:板蓝根提取液对11株细胞的相对细胞相对增殖率无显著性差异;对MDCK,MDCK2,A549,HepG2的细胞最高相对增殖率分别为142. 06%,144. 42%,146. 66%,130. 05%;增强MDCK细胞的Erk磷酸化水平;使用Erk抑制剂可有效降低板蓝根提取液致MDCK的增殖能力。结论:板蓝根提取液可以选择性提高上皮来源或具有上皮细胞特性的细胞株(MDCK,A549,HepG2)的增殖能力,不影响其他来源的细胞增殖能力。 Objective:To evaluate the effects of Radix Isatidis extraction on cell proliferation and the associated mechanisms.Methods:MTT and CCK-8 assays were used to determine the effects of Radix Isatidis extraction on cell survival and relative proliferative activities in 14 cell lines with different source.Western blotting was used for assaying the enhancement of p-Erk of MDCK cell line after treated by Radix Isatidis extraction.The inhibitor of Erk was used for assaying the cell survivals activities of MDCK cell line after treated with Radix Isatidis extraction.Results:In 11 cell lines treated by Radix Isatidis extraction,no significant difference was found in cell proliferation rate.In MDCK,MDCK2,A549 and HepG2 cells,the relative proliferation rates were highest(142.06%,144.42%,146.66%,130.05%,respectively).Radix Isatidis extraction significantly enhanced the level of Erk phosphorylation in MDCK cells.Erk inhibitor effectively reduced the proliferation of MDCK cells which induced by Radix Isatidis extraction.Conclusion:Radix Isatidis extraction can selectively improve the proliferation ability of epithelial derives or epithelial cell lines(MDCK,MDCK2,HepG2,A549),but not affect the cell proliferation ability of other cell derives.
作者 吴彦霖 张媛 刘俊 罗剑 高华 WU Yan-lin;ZHANG Yuan;LIU Jun;LUO Jian;GAO Hua(National Institutes for Food and Drug Control,Beijing102629,China;PLA Institute for Drug and Instrument Control,Beijing100071,China)
出处 《中国新药杂志》 CAS CSCD 北大核心 2019年第17期2135-2140,共6页 Chinese Journal of New Drugs
基金 国家药典委员会国家药品标准提高研究(670) 国家自然科学基金(81303194)
关键词 细胞增殖 上皮细胞 板蓝根 丝裂原活化蛋白激酶 细胞外调节蛋白激酶磷酸化 cell proliferation epithelial cell Radix Isatidis mitogen-activated protein kinase(MAPK) p-Erk
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