大孔吸附树脂纯化断血流总皂苷工艺研究

Studies on the Process of Herba Clinopodii Saponins Purified with Macroporous Adsorption Resin

目的:研究大孔吸附树脂纯化断血流总皂苷的工艺条件及参数。方法:以洗脱率、精制度等为指标,考察大孔吸附树脂对断血流总皂苷的吸附性能和洗脱参数。结果:11.4m l断血流总皂苷样品液(生药0.2g/m l)上大孔吸附树脂(Φ15mm×H90mm,干重2.5g),用蒸馏水,30%、70%乙醇各3BV依次洗脱,断血流总皂苷富集于70%乙醇洗脱液部位。结论:采用大孔吸附树脂技术富集、纯化断血流总皂苷,其洗脱率为86.8%,精制度为153.2%,该法可较好地纯化断血流总皂苷。

Objective:To study the technological parameters of the purification process of saponins with macroporous adsorption resin.Methods:The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers.Results:11.4ml of the extraction of Herba Clinopodii(crude drugs 0.2g/ml) was purified with a column of macroporous adsorption resin(Φ15mm×H90mm,dry weight 2.5g) and washed with 3BV of distilled water,then eluted with 3BV of 30% ethanol and 3BV of 70% ethanol.Most of saponins were collected in the 70% ethanol.Conclusion:With macroporous adsorption resin adsorbing and purifying,the elutive ratio of saponins is 86.8% and the purity reachs 153.2%.So this process of applying macroporous adsorption resin to adsorb and purify Saponins is feasible.