柳叶蜡梅对SD大鼠致畸作用研究

A study on teratogenic effects of Chimonanthus salicifolius S. Y. Hu extract on SD rats

目的研究柳叶蜡梅水提取浓缩液对SD大鼠母体毒性和胚胎的致畸性。方法交配成功的SD雌鼠64只,随机分为柳叶蜡梅低、中、高剂量组及阴性对照组,采用水提法制备柳叶蜡梅浓缩液,低、中、高剂量组孕鼠分别于妊娠7~16 d连续灌胃给予受试物,每天1次;阴性对照组给予蒸馏水。记录孕鼠孕期一般状况和体重变化,妊娠20 d后剖检孕鼠,检查胎仔数及胎仔形态的变化。结果低、中、高剂量组和对照组大鼠受孕率、孕期总增重、吸收胎鼠数及死胎鼠数比较,差异均无统计学意义(P>0.05)。四组胎鼠肋骨数目、胸骨数目、第五块胸骨呈点状数目及头顶骨骨化不全数目比较,差异均无统计学意义(P>0.05)。结论在本实验剂量和条件下,未见柳叶蜡梅水提取浓缩液对大鼠有母体毒性、胚胎毒性和致畸性。

Objective To study maternal toxicity,embryotoxicity and teratogenecity of Chimonanthus salicifolius S. Y.Hu in SD rats.Methods A total of 64 successfully mated female SD rats were randomly divided in to 4 groups(16 per group),in which 3 experimental groups were daily treated with 3.75,7.5 and 15.0 g/kg. bw test substance by lavage from7 th to 16 th day during gestation respectively. Body weight and general conditions of the pregnant rats were recorded during the study. On the 20 th day in pregnancy,the rats were anatomized and examined grossly,the fetuses were removed and counted,weight,length,visceral and skeletal changes were then examined. Results There was no significant difference in the conception rate,total weight gain during the pregnancy and the number of living, dead and resorbed fetuses between each dosage groups and the control group(P>0.05). The number of the rib, sternum, the fifth sternum punctated and the parietal bone which were ossified defectively all showed no difference among the four groups(P>0.05). Conclusion Chimonanthus salicifolius S. Y. Hu extract had no obvious maternal toxicity,embryotoxicity and teratogenecity in SD rats under this experiment condition.