梓醇配伍大黄素对EAE小鼠大脑内NRG1的影响

Effects of Catalpol and Emodin on NRG1 in EAE Mice Brain

目的:观察梓醇配伍大黄素对EAE小鼠大脑内NRG1表达的影响,讨论其减轻髓鞘损伤及其修复机制。方法:C57BL/6j小鼠背部脊柱旁皮下4点注射MOG35-55诱导产生EAE,观察EAE小鼠神经功能缺损严重程度,于第18天及第40天取小鼠大脑组织,H&E染色观察其炎性浸润,透射电子显微镜观察小鼠大脑髓鞘、轴突病理改变,Realtime rt-PCR和WB检测小鼠大脑NRG1基因及蛋白的表达水平。结果:梓醇配伍大黄素能够改善EAE小鼠临床症状和神经功能评分,配伍组与模型组比较神经功能损伤评分降低,差异有统计学意义。H&E染色显示各治疗组炎性浸润均有所减轻,TEM显示各治疗组髓鞘环形层状结构松散变形程度均较模型组减轻,WB、Realtime rt-PCR结果显示配伍组NRG1基因及蛋白表达水平较正常组及模型组明显升高。结论:梓醇配伍大黄素能减轻EAE小鼠病理改变,减缓炎症进程,增加NRG1在大脑内的表达,对髓鞘的再生具有一定的促进作用。

To observe the effect of catalpol combining with emodin on the expression of neuregulin-1(NRG1)and in the brain of experimental autoimmune encephalomyelitis(EAE)mice.Methods:EAE model were induced by subcutaneous injection of antigens MOG35-55,and neurological scores were estimated daily.The pathological changes of the brain were observed by H&E staining on the day 18 and 40.Pathological changes of myelin and axon were observed by transmission electron microscope(TEM).Real-time reverse transcription-polymerase chain reaction(realtime rt-PCR)and Western blot(WB)were used to detect the expression of NRG1 mRNA and protein in mice brain.Results:Catalpol&Emodin could alleviate neurological dysfunction and catalpol&emodin group had lower scores comparing with model group on the day 18 and 40.H&E staining showed that the inflammatory infiltration of each treatment group was alleviated;TEM showed that the loose deformation of the myelinated annular lamellar structure in each treatment group was alleviated compared with the model group;The NRG1 gene and NRG1 protein levels were significantly upregulated in catalpol&emodin group comparing with normal group and model group.Conclusion:Catalpol&emodin could alleviate the inflammating and pathological changes and also promote the NRG1 expression in the EAE mice.