食源性沙门菌的分离鉴定及血清分型能力验证

Isolation and identification of foodbrone Salmonella and serotyping in proficiency verification

目的验证本实验室在国家食品安全风险监测中检测沙门菌的能力及实验室的质量控制水平。方法按照考核方作业指导书对实验样品进行前处理,参照GB 4789.4-2016《食品安全国家标准食品微生物学检验沙门氏菌检验》进行沙门菌的检验,将全自动微生物生化鉴定仪鉴定为沙门菌的阳性菌株进行血清学鉴定和分型。结果 1号和3号样品在沙门氏+菌显色平板等3种选择性分离平板上均长出典型沙门菌特征的菌落,2号样品在3种分离平板上均无菌落生长;1号和3号样品中的可疑菌落经生化鉴定后结果与阳性对照菌株的一致,均符合沙门菌典型的生化反应特征;通过对可疑沙门菌进行血清分型,1号样品血清鉴定结果为肯塔基沙门菌,3号样品血清鉴定结果为鼠伤寒沙门菌,2号样品中未检出沙门菌。3个样品的能力验证结果与考核方一致,本实验室取得满意结果。结论参加能力验证是提高检验检测机构实验室质量控制最有效的手段之一,通过参加本次能力验证,能有效提高本实验室出具检测数据的准确性和可靠性,同时也能有效提升本实验室从事食品检验的能力和质控水平。

[Objective]To verify the capacity for Salmonella detection and laboratory quality control in national food safety risk monitoring project of China of the laboratory.[Methods]The experimental samples were processed in accordance with the requirements of operation instruction.The detection was performed according to the GB 4789.4-2016 of National food safety standard-Food microbiological examination-Examination of Salmonella.The positive strains of Salmonella identified by automatic microbiological biochemical assay were analyzed for serological identification and classification.[Results]In sample 1 and sample3,the typical salmonella colonies were grown on three selective separation plates,such as Salmonella+colouration flat plate.There was no colony growth on these separation plates in sample 2.After biochemical identification,the results of the suspicious colonies of sample 1 and sample 3 were consistent with those of the positive control strains,and were consistent with the typical biochemical characteristics of Salmonella.By serotypying on suspected salmonella,sample 1 was identified as Salmonella Kentucky,sample 3 was identified as Salmonella typhimurium,and sample 2 was not detected any Salmonella.The results of the three samples were satisfactory,which were consistent with the organizer’s results.[Conclusion]Participating in capacity verification is one of the most effective means to improve laboratory quality control.Through this proficiency verification,the accuracy and reliability of the test data issued by our laboratory are effectively improved,and the ability and quality control level of the laboratory to conduct food inspection are also effectively improved.