基于氧化应激探讨针刺人迎穴的降压效应

Study on the Hypotensive Effect of Acupuncture on Renying Point Based on Oxidative Stress

目的观察针刺人迎穴对大鼠血压及总抗氧化能力(T-AOC)的影响,并从抗氧化应激角度探讨其降压效应。方法将18只SHR大鼠随机分为模型组、毫针组、电针组各6只,WKY大鼠6只为空白组。毫针组取双侧人迎穴,不予任何手法;电针组在此基础上加用电针1 min,均留针20 min。模型组及空白组只予抓取固定20 min。4组均每日上午干预1次,连续4周。分别于干预前、干预后2周末、4周末测量血压,4周后取外周血及延髓,分别检测T-AOC在血清中的含量及延髓中的蛋白表达。结果毫针组SBP、DBP干预2周后与干预前对比,无统计学意义(P>0.05);干预4周后较干预前可见明显下降(P<0.05)。电针组SBP、DBP干预2周后、4周后分别与干预前对比,均可见明显下降(P<0.05)。电针组干预4周后的SBP与同节点毫针组对比,差异有统计学意义(P<0.05);同节点两组间DBP比较无统计学意义(P>0.05)。血清中T-AOC含量及延髓中T-AOC蛋白表达,毫针组、电针组分别与模型组对比,均可见明显提高(P<0.05);毫针组与电针组对比,无统计学意义(P>0.05)。结论针刺人迎穴能有效降低血压,加用电针则起效更快,疗效更著,且对收缩压的改善更为明显。同时亦能明显提高机体的抗氧化活性,改善氧化应激,从而在降压的同时发挥对心脑血管的保护作用。

Objective To observe the influence on rat’s blood pressure and T-AOC treated with acupuncture in Renying(ST9),and discuss the pressure reduction effect from the anti-oxidative stress point.Methods 18 Spontaneous Hypertension Rats(SHR) are randomly divided into 3 groups:model group,treatment group with acupuncture,treatment group with electro-acupuncture,each group has 6 SHR rats.6 Wistar-Kyoto(WKY) rats as the blank group.The treatment group with acupuncture stimulate Renying(ST9) without any needling manipulation bilaterally;base on that,the treatment group with electro-acupuncture add electro-acupuncture for one minute,both of them retaining needle for 20 minutes.The model group and the blank group only need to be caught and fixed for 20 minutes.The 4 groups are all be intervened at forenoon each day and last for 4 weeks.Their BP all need to be measured before intervention,and after i ntervention every 2 weeks,and get their blood and medulla oblongata to detect the content and protein expression of T-AOC.Results There has no statistical difference between before and 2 weeks after the intervention of SBP and DBP of treatment group with acupuncture(P>0.05),but there has significant reduction between before and 4 weeks after the intervention(P<0.05).There has significant reduction of SBP and DBP of treatment group with electro-acupuncture compared 2 weeks later and 4 weeks later to before the intervention(P<0.05).SBP between treatment group with acupuncture and treatment group with electro-acupuncture have significant difference when 4 weeks after the intervention(P<0.05),but there is no statistical difference of DBP(P>0.05).The conte nt in serum and protein expression in medulla of T-AOC,there has significant improvement compered treatment group with acupuncture and treatment group with electro-acupuncture to the model group(P<0.05),but there has no statistical difference between treatment group with acupuncture and treatment group with electro-acupuncture(P>0.05).Conclusion Acupuncture in Renying(ST9) is effective in lowering blood pressure,and it is more effective and obvious to use electric acupuncture,especially in the reduction of SBP.At the same time,it can obviously raise the antioxidant activity and improve the oxidative stress,so as to play the protective role of the cardiovascular and cerebrovascular system.