哈蟆油对抑郁模型小鼠行为学影响的研究

Effect of Ranae Oviductus on the Behavior of Depression Model Mice

目的考察哈蟆油对慢性温和不可预知应激所致抑郁模型小鼠行为学的影响。方法 42只健康雄性小鼠,随机分为空白组、模型组、氟西汀组(3 mg/kg)、哈蟆油高剂量组(800 mg/kg)、哈蟆油中剂量组(400 mg/kg)、哈蟆油低剂量组(200 mg/kg),通过每日随机给予模型动物1~2种不同的刺激,连续造模42 d,每周称量1次体质量,第28天开始给药,连续给药14 d。于第28天、第42天通过悬尾实验、强迫游泳实验、旷场实验对各组小鼠进行行为学评价。结果造模28 d后,除空白组外,各组小鼠均出现明显的体质量下降;悬尾实验、强迫游泳实验各组小鼠不动时间增加;旷场实验运动时间、运动路程、运动速度、中央区活动时间下降,角落活动时间增加(P <0.01或P <0.05),给药14 d后,与模型组相比,各组小鼠抑郁行为均出现明显改善(P <0.01或P <0.05)。结论综合上述结果,哈蟆油可以改善由慢性不可预知温和应激所致小鼠的抑郁行为。

Objective To investigate the effect of Ranae Oviductus on the behavior of mice with depression induced by chronic mild unpredictable stress.Methods 42 healthy male mice were randomly divided into Blank group,Model group,Fluoxetine group(3 mg/kg),Ranae Oviductus high dose group(800 mg/kg),Ranae Oviductus medium dose group(400 mg/kg),Ranae Oviductus low dose group(200 mg/kg),by randomly giving the model animals 1 to 2 different stimuli every day,the model was built continuously for 42 days,and the body weight was weighed once a week,dosing started on the 28 th day,continuous administration for 14 days.On the 28 th and 42 nd days,the behavior of each group of mice was evaluated by tail suspension experiment,forced swimming experiment and open-field experiment.Results After 28 days of modeling,all groups of mice showed significant weight loss except for the blank group;suspension test,forced swimming test static time increased;in the open field experiment,the exercise time,the movement distance,the movement speed,the activity time in the central area decreased,and the corner activity time increased(P<0.01 or P<0.05),after 14 days of administration,compared with the Model group,the depression behavior of each group showed significant improvement(P<0.01 or P<0.05).Conclusion Based on the a bove conclusions,Ranae Oviductus can improve the depression behavior of mice caused by chronic unpredictable mild stress.