针刺列缺穴对过敏性鼻炎模型大鼠鼻黏膜病理学的影响

Exprimental Research on the Effect of Acupuncturing at Lieque on the Nasal Mucosa Pathology Changes of Model Rats with Allergic Rhinitis

目的观察针刺列缺穴对过敏性鼻炎大鼠行为学和鼻黏膜病理学的影响。方法卵白蛋白建造大鼠过敏性鼻炎模型。60只SD雄性健康大鼠随机分为空白组、对照组和针刺组每组各20只。观察针刺列缺穴对大鼠行为学和病理学的影响。结果实验前(第1日)3组行为学差别不大(P>0.05);第7日(模型复制局部激发结束后),与空白组比较,对照组、针刺组18只大鼠鼻痒、喷嚏和流涕等行为学总积分增加(P<0.01);第14日(干预后),针刺组行为学总积分与模型组比较明显降低(P<0.05)。空白组大鼠鼻黏膜肉眼观无充血、无肿胀,颜色淡红;光镜下无嗜酸性粒细胞浸润;对照组大鼠鼻黏膜肉眼观苍白,可见多处溃疡,充血水肿,鼻甲肿胀,时有清稀分泌物,光镜下腺体增生,嗜酸性粒细胞浸润明显增多;针刺组鼻黏膜充血、水肿、溃疡较对照组明显好转,且嗜酸性粒细胞数目较对照组明显减少。结论针刺列缺可加快修复鼻黏膜上皮细胞,减少嗜酸性粒细胞等炎症细胞浸润而改善过敏症状。

Objective: To observate the AR rat′s ethology and nasal mucosa pathology changes after acupuncturing at Lieque. Methods: 60 SD healthy male rats were randomly divided into the blank group,the model group and the acupuncture group,built with the model of allergic rhinitis. Results: Bofore experiment,there were no significant behavioral differences in the three groups(P > 0.05). On the 7th day,compared with the blank group,18 rats in the control group and the acupuncture group had an increasing general behavior score,such as nasal itching,sneezing and runny nose(P < 0.01). On the 14 th day,after acupuncturing at Lieque,compared with the model group,the general behavior score of the acupuncture group had an obvious reduce(P < 0.05). In the blank group,there was no obvious congestion and swelling in nasal mucosa with pink color and no eosinophilic infiltration under mircoscope. In the control group,there were obvious pale nasal mucosa,ulcers,congestion,swelling,Turbinate swelling,sometimes clear dilute secretion,gland hyperplasia under microscope,and an increase of eosinophilic infiltration. In the acupuncture group,nasal mucosa congestion,edema and ulcers were improved obviously,and the number of EOS was significantly reduced. Conclusion: Acupuncturing at Lieque can speed up the repair of nasal mucosa,decrease EOS and improve the symptoms of allergy.