丹参酮ⅡA磺酸钠对大鼠急性心肌梗死模型心脏做功的影响

Effect of Sodium Tanshinone ⅡA sulfonate on Cardiac Work in Rat Model with Acute Myocardial Infarction

目的观察丹参酮ⅡA磺酸钠对急性心肌梗死动物模型大鼠离体心脏做功的影响。方法大鼠40只,按随机数字表法分为模型组、心得安组、异丙肾上腺素组、丹参酮组,采用舌下静脉注射垂体后叶素的方法建立大鼠急性心肌梗死动物模型,于造模成功后断头取心并固定于Langendorff离体心脏灌流装置上灌流给药,分别测定各组离体心脏在药前10 min及药后0.5 h、1.5 h、3 h 4个时间点的左室最大上升/下降速率(±LVdp/dtmax)、心率(HR)、心肌收缩力(IT)、左室内压力峰值(LVSP)、左室射血分数(LVEF)等,籍此评价丹参酮ⅡA磺酸钠对大鼠急性心肌梗死离体心脏做功的影响。结果与给药前及心得安组比较,丹参酮ⅡA磺酸钠组在0.5 h、1.5 h两时间点±LV dp/dtmax、IT、LVSP、LVEF明显增高(P<0.05),但对HR无明显影响。结论丹参酮ⅡA磺酸钠有类似于β-受体激动剂异丙肾上腺素增强心肌收缩力的药理效应,其作用机制有可能是减轻心肌细胞及冠状动脉平滑肌钙超载,扩张冠状动脉、避免由钙超载诱导心肌梗死、提高急性心肌梗死模型大鼠离体心脏做功从而起到一定的心肌保护作用。

Objective: To observe the effect of sodium tanshinone Ⅱ A sulfonate on cardiac work in rat model with acute myocardial infarction. Methods: 40 rats were randomly divided into the model group,propranolol group,isoproterenol group,tanshinone group. the method of sublingual vein injection of pituitrin was adopted to establish model of acute myocardial infarction in rats. After the success in modeling,the brains were removed and the hearts were fixed on Langendorff isolated heart perfusion device for perfusion administration. The following data was measured 10 min before administration and after administration of 0.5 h,1.5 h,3,such as maximum rise /fall rate(+ LVdp/dtmax) in left ventricular heart in vitro,heart rate(HR),myocardial contractility(Isometric tension,IT),left ventricular pressure peak(LVSP),left ventricular ejection fraction(LVEF),and so on,to evaluate the effect of sodium tanshinoneⅡA sulfonate on cardiac work in rat model with acute myocardial infarction. Results:Compared with before dosing and propranolol group,±LV dp/dtmax,IT,LVSP and LVEF of tanshinone Ⅱ A sulfonate group from 0.5 h to 1.5 h increased significantly(P < 0.05),but had no obvious effect on HR. Conclusion:Sodium tanshinone ⅡA sulfonate has a pharmacological effect on enhancing myocardial contractile force,similar to β-receptor agonist: isoproterenol. Its mechanism may be to reduce calcium overload in cardiac muscle cells and coronary artery smooth muscle cells,dilate coronary artery,avoid myocardial infarction induced by calcium overload,improve cardiac work in rat model with acute myocardial infarction,so as to play a certain role in myocardial protection