肿瘤抗巨噬细胞移动因子的生物学特性及用于肿瘤诊断

Biological Studies and Clinical Application of Anti-migration Factor in Tumor Diagonosis

观察肿瘤抗巨噬细胞移动因子(Anti-Migration Factor,AMF)在体内、外对Mφ的作用。结果,带瘤小鼠腹腔Mφ及周血单核细胞的移动较正常低下;正常小鼠腹腔Mφ在体外经AMF作用后,贴壁能力减弱且移动明显缺陷。带瘤动物在可见的实体瘤及腹水症象(腹水瘤)出现以前血清AMF就呈阳性。对134例未经治疗的各种肿瘤患者进行双盲检测,血清AMF阳性率达88.9％;而125例正常人及11例住院非肿瘤病人血清均为阴性,提示该方法可用于肿瘤诊断。

Mφ in peritoneal cavity and monocyte in blood of mice with tumor show poor migration in vitro than that of normal mice. Normal Mφ treated with AMF (from S180 ascites) in vitro also show poor migration. Tumor AMF appears in sera before detection of solid tumor or sign of ascites. Sera from 134 cases of tumor patients were assayed (double-blind)by Mφ inhibition test with a positive rate of 88.9%, while that of sera from 125 normal person and 11 cases of non-tumor patients showed negative result, suggesting that the test can be used for tumor diagnosis.