前列腺素E对人B细胞增殖的抑制

Suppression of Human B Cell Proliferation by PGE

单核细胞对人淋巴细胞反应既可起增强作用,又可起抑制作用。有实验报道,前列腺素E（PGE）能抑制抗原或丝裂素诱导的T细胞增殖及白细胞介素2（IL-2）等淋巴因子的产生,也可抑制葡萄球菌诱导人B细胞增殖与分化。我们发现单核细胞和外源性PGE1或PGE2能抑制anti-μ（羊抗人IgM重链抗体的F（ab1）2片段）诱导人B细胞的增殖,PGE生物合成抑制剂消炎痛可消除其抑制作用。从而表明PGE在单核细胞抑制效应中的介导作用。为进一步证明其作用。

In our previous experiments, it was observed that the proliferation of human B lymplocytes induced by large dose anti-μ (100μg/ml) was suppressed by autologous or allogenic monocytes. Since indomethacin can mitigate the suppressive effect of monocytes and exogenous PGE1 or PGE2 can also inhibit the B cell proliferation induced by anti-μ, it is concluded that the suppressive effect of monocytes is at least partially mediated by PGE. In order to further confirm the conclusion, PGE2 produced in the supernatant of cell culture was detected by RIA (radioimmunoassay) . At the same time 3H-TdR uptake was . assessed in separate cell cultures set up in the same condition. The results indicate that with the increase of monocyte addition into cell culture, 3H-TdR uptake by B cells was found to be decreased with increase of PGE2 produced in the supernatant. Besides, indomethacin was found to antagonize suppressive effect of monocytes and inhibit the production of PGE2.