甘西鼠尾草水溶性多糖提取工艺的优化及体外抗氧化活性测定

Optimization of Extraction Process and Antioxidative Activity on Polysaccharides of Salvia przewalskii

本文利用超声波辅助水提醇沉法提取甘西鼠尾草粗多糖,应用单因素和Box-Behnken响应面法进行提取工艺优化.对甘西鼠尾草粗多糖进行脱色素、脱蛋白处理得到初步纯化多糖,并对粗多糖和初步纯化多糖进行体外抗氧化活性的测定.结果表明,甘西鼠尾草粗多糖的最佳提取条件为:液料比31:1 m L/g、提取时间2. 7 h、提取温度75℃、超声波功率300 W.在此条件下,甘西鼠尾草粗多糖的提取率为6. 568%,与预测值基本一致.清除自由基实验表明,在一定浓度范围内,对·OH自由基清除活性最高清除率达64. 34%;对DPPH清除活性最大清除率达83%.

OBJECTIVE: To optimize the extraction process of water-soluble polysaccharides from Salvia przewalskii and to explore the antioxidant activity of polysaccharides from S. przewalskii.METHOD: Ultrasonic-assisted water extraction was used to extract the crude polysaccharide from S.przewalskii( GXSWCc) and the response surface methodology was used to optimize the extraction process. The crude polysaccharides were de-pigmented and deproteinized to obtain preliminary purified polysaccharides,and the antioxidant activities of crude polysaccharides and preliminary purified polysaccharides were determined in vitro. RESULT: The optimum extraction conditions of GXSWCc were as follows: liquid/solid ratio was 31: 1 m L/g,extraction time was 2. 7 h,extraction temperature was 75°C,ultrasonic power was 300 W. Under this condition,the polysaccharide extraction rate of S. przewalskii was 6. 568%,which was basically consistent with the predicted value. Free radical scavenging experiments showed that the highest scavenging rate of OH radical scavenging activity was64. 34% and DPPH scavenging actirity was 83% with in a certain concentration range.