Changes in mass spectrometry of proteins in patients with 1-bromopropane poisoning after treatment

Objective To investigate the changes in mass spectrometryof proteins in patients with 1-bromopropane(1-BP) poisoning after treatment and their biologicalfunctions. Methods From May 2016 to December 2017,3 male patients aged 31-47 years with 1-BP poisoning inBao'an District of Shenzhen,China,were enrolled in thisstudy. The whole blood sample (2 ml) was collected beforeand after treatment. Label-free mass spectrometrybasedproteomics was used for protein identification andquantification. The differentially expressed proteins aftertreatment were analyzed. Bioinformatics tools were usedto analyze the functions of the identified proteins and thebiological processes involved in. Results Proteomic analysisshowed that there were 47 proteins that were differentiallyexpressed more than 2-fold ( P < 0. 05) aftertreatment in the patients with 1-BP poisoning;27 wereup-regulated and 20 were down-regulated in the serum oftreated patients.