Effects of 3-methyladenine on airway inflammation,airway hyperresponsiveness and mucus secretion in asthmatic mice

Objective To investigate the effects of 3-methyladenineon airway inflammation,airway hyperresponsivenessand mucus secretion in asthmatic mice,and to explore itsmechanism. Methods C57BL /6J female mice were randomlydivided into normal control group ( PBS),OVAgroup(OVA),OVA with 3-methyladenine group (OVA +3-MA),and OVA with 4-phenylbutyrate group (OVA +4-PBA). OVA group,OVA + 3-MA group and OVA + 4-PBA groups were all sensitized and challenged with OVAto establish asthmatic models,while PBS group was givenPBS as a control. At 2 h before challenge,OVA + 3-MAgroup was intraperitoneally injected with 3-methyladenine,and OVA + 4-PBA group was intraperitoneally injectedwith 4-phenylbutyrate. Airway hyperresponsiveness,eosinophils,and pathological changes of pulmonarytissue (hematoxylin-eosin,HE staining) were measuredto confirm the establishment of asthmatic models.