摘要
Objective To investigate the effects of artesunatetreatment on chronic graft-versus-host disease(cGVHD). Methods Recipient BALB /c mice received8 × 106 bone marrow cells with 8 × 106 spleen cells fromB10D2 mice. Artesunate solubilized in acetone was injectedintraperitoneally every day at a dose of 1 mg /kg onDay 28 after BMT. The clinical scores,survival and histopathologicaldamage were analyzed. The frequency ofTh17 and Tregs in PB and spleens from the mice wereevaluated by flow cytometry. In addition,CD4 + T cellsfrom the spleens of mice were cultured in vitro,thenstimulated with artesunate,the frequency of Th17 andTregs in these splenocytes were evaluated by flow cytometry.
