摘要
目的:采用HPLC-UV法测定人脑脊液中万古霉素浓度为颅内感染患者调整剂量提供依据,探讨颅内感染患者鞘内注射万古霉素的适宜给药方案。方法:以外标法定量,采用Waters XBridge C_(18)(150 mm×4.6 mm,5μm)色谱柱,在流动相为乙腈-磷酸二氢钾缓冲液(8.5∶91.5,v/v)、柱温为25℃、流速为1.0 ml·min^(-1)、检测波长为236 nm条件下检测人脑脊液中万古霉素的浓度,进行方法学考察后将其应用于临床。结果:人脑脊液中万古霉素1.0~100.0μg·ml^(-1)时与峰面积之间呈良好线性关系(r^2=0.999 8);日内精密度RSD<2.68%,日间精密度RSD<4.86%,绝对回收率为94.23%~96.7%。室温下放置24 h,冻融循环3次和冰冻30 d的稳定性均良好。结论:所建立的方法简便、准确度高、特异性强,适用于临床人脑脊液中万古霉素浓度检测,可为颅内感染患者个体化、精准化治疗提供依据。
Objective: To determine the vancomycin concentration in human cerebrospinal fluid by HPLC-UV to provide basis for dose adjustment in patients with intracranial infection. Methods: The quantification was carried out by an external standard method,a Waters XBridge C18 column (150 mm× 4.6 mm,5 μm) was used,and the mobile phase consisted of acetonitrile and potassium dihydrogen phosphate (8.5 ∶ 91.5,v/v) at the flow rate of 1.0 ml·min-1. The column temperature was maintained at 25℃. The detection wavelength was 236 nm. Results: The good linear relationship was obtained between the peak area and the vancomycin concentration from 1.0 to 100.0 μg·ml-1 in human cerebrospinal fluid (r2= 0.999 8). The RSD of intra-day precision was less than 2.68%,the RSD of inter-day precision was less than 4.86%,and the absolute recovery was 94.23%-96.7%. The samples of vancomycin in human cerebrospinal fluid were all stable at room temperature for 24 h,after three freeze-thaw cycles and at-80℃ for 30 days. Conclusion:The established method is simple,accurate and specific,and suitable for the detection of vancomycin concentration in clinical human cerebrospinal fluid,which provides basis for the individualized and precise treatment of patients with intracranial infection.
作者
缪文清
汪硕闻
范先煜
石卫峰
李琴
范国荣
Miao Wenqing;Wang Shuowen;Fan Xianyu;Shi Weifeng;Li Qin;Fan Guorong(Department of Clinical Pharmacy,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China)
出处
《中国药师》
CAS
2019年第3期399-403,共5页
China Pharmacist
基金
国家自然科学基金项目(编号:81703597)
上海市卫计委重要薄弱学科建设项目(编号:2016ZB0302)