HPLC法测定氧化白藜芦醇的含量

Determination of Oxyresveratrol by HPLC

目的:建立高效液相色谱法测定氧化白藜芦醇含量的方法。方法:采用Agilent TC-C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(30∶70)为流动相,流速为1.0 ml·min^(-1),柱温为30℃,检测波长为329 nm,进样量为20μl。结果:在光照、高温、氧化、酸碱破坏下,分离度良好,氧化白藜芦醇在19.28～192.88μg·ml^(-1)范围内呈良好线性关系(r=0.999 7)。结论:本法操作简便,灵敏度高,重复性好,可有效控制氧化白藜芦醇质量。

Objective: To establish an HPLC method for the determination of oxyresveratrol. Methods: An Agilent TC-C18 column (250 mm×4.6 mm,5 μm) was used,the mobile phase consisted of acetonitrile-water (30 ∶ 70),the flow rate was 1.0 ml·min-1,the temperature of column was set at 30℃,the detection wavelength was set at 329 nm,and the sample size was 20 μl. Results: With illumination,high temperature,oxidation,acid and alkali stressing trial,the method showed good separation,and oxyresveratrol had a good linear relationship within the range of 19.28-192.88 μg·ml-1 (r= 0.999 7). Conclusion: The method is simple and sensitive with good repeatability,and can effectively control the quality of oxyresveratrol.