HPLC法同时测定毛连菜中咖啡酸和木犀草苷含量

Simultaneous Determination of Caffeic Acid and Luteoloside in Picris hieracioides L. by HPLC

目的:建立高效液相色谱法(HPLC)同时测定毛连菜中咖啡酸和木犀草苷含量的方法。方法:采用Waters Sun Fire C_(18)色谱柱(250 mm×4.6 mm 5μm),流动相为乙腈-0.1%磷酸溶液梯度洗脱,检测波长323 nm,流速1.0 ml·min^(-1),进样量10μl,柱温30℃。结果:咖啡酸和木犀草苷的色谱峰可较好地分离,咖啡酸浓度在1.014～40.560μg·ml^(-1)(r=0.999 5)、木犀草苷浓度在2.006～80.240μg·ml^(-1)(r=1000 0)范围内与峰面积呈良好的线性关系;平均加样回收率分别为92.84%,92.62%,RSD分别为2.0%,1.93%(n=6)。结论:该方法准确性、重复性、专属性较好,可用于毛连菜的质量控制。

Objective: To develop an HPLC method for the content determination of caffeic acid and luteoloside in Picris hieracioides L.. Methods: A Waters Sun Fire C18 chromatographic column (250 mm×4.6 mm 5 μm) was used with the mobile phase of acetonitrile-0.1% phosphate solution at a flow rate of 1.0 ml·min-1,the column temperature was 30℃,the detection wavelength was323 nm,and the injection volume was 10 μl. Results: The separation of caffeic acid and luteoloside was good. The linear range of caffeic acid and luteoloside was 1.014-40.560 μg·ml-1 (r= 0.999 5) and 2.006-80.240 μg·ml-1 (r= 1.000 0),respectively. The mean recovery was 92.84% (RSD = 2.0%) and 92.62% (RSD = 1.93%) (n = 6),respectively. Conclusion: The method has good accuracy,repetitiveness and specificity,which can be used for the quality control of Picris hieracioides L..