摘要
以白杨素含量为指标,采用L9 (34)正交试验,考察提取溶媒、总液料比、提取次数和提取时间对经石油醚冷浸脱脂后的杨树皮总酚酸提取率的影响,优化提取工艺。最佳提取工艺为60%乙醇,总液料比10∶1,回流提取3次,每次2 h。本提取工艺方法简单、合理,是杨树皮中总酚酸的最佳提取工艺。
With the content of chrysin as index,the effects of ethanol concentration,the total liquid-solid ratio,repeated times and extraction time on the extraction rate of total phenolic acids from the barks of P.canadensis after degreasing bycold maceration with petroleum ether were investigated by the orthogonal experimental design using L9(34),to optimize the extraction process.The best extraction process was as following:ethanol concentration,60%;the total liquid-solid ratio,10∶1(mL·g-1);repeated extracting 2 h for three times.The extraction method is simple and feasible,and is the best extraction process of total phenolic acids from the barks of P.canadensis.
作者
王碧
严奇
赵友谊
王鸣
刘飞
廖盼华
王奇志
Wang Bi;Yan Qi;Zhao Youyi;Wang Ming;Liu Fei;Liao Panhua;Wang Qizhi(Jiangsu Key Laboratory for the Research and Utilization of Plants Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Jiangsu Nanjing 210014,China;The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China)
出处
《中国野生植物资源》
CSCD
2019年第4期13-16,共4页
Chinese Wild Plant Resources
基金
江苏省科技计划社会发展-面上项目(BE2016753)
关键词
加拿大杨
酚酸
白杨素
正交试验法
紫外分光光度法
Populuscanadensis moench
Phenolic acids
Chrysin
Orthogonal experimental design
Ultraviolet spectrophotometry
