Th22和Th17细胞在白癜风病理机制中的作用

The Effects of Th22 and Th17 in the Pathogenesis of Vitiligo

目的探讨Th22和Th17细胞可能在白癜风发生发展中的作用机制。方法选择白癜风患者60例(按不同分期划分,活动期白癜风患者30例,静止期白癜风患者30例;按不同分型划分,寻常型白癜风患者29例,节段型白癜风患者31例),以年龄和性别匹配的健康正常人30例为对照。取外周静脉血,免疫荧光PCR法检测外周血中IL-22mRNA和IL-17mRNA表达,酶联免疫吸附法(ELISA)检测IL-22和IL-17表达含量。将所检测结果与白癜风患者病情进行相关分析。结果 (1)白癜风患者实验组外周血中IL-22mRNA和IL-17mRNA以及IL-22和IL-17蛋白的表达水平均明显高于健康对照组,其中活动期白癜风患者明显高于静止期患者。(2)寻常性白癜风患者外周血中IL-22mRNA和IL-17mRNA以及IL-22和IL-17表达水平显著高于节段型患者。(3)在白癜风患者组中,外周血IL-22mRNA和IL-17mRNA表达水平以及IL-22和IL-17水平呈正相关(r=0. 753,P=0. 000)。结论 Th22和Th17细胞在白癜风发生发展中起到一定作用。

Objective To explore the possible mechanisms of Th22 and Th17 cells in the pathogenesis and development of vitiligo. Methods We collected 60 cases of patients with vitiligo diseases( according to different stages,30 case of active patients,30 cases of stationary patients;according to different types,29 cases of ordinary patients and 31 cases of segmental patients). A total of 30 cases of healthy people as normal control,whose age and sex matched with patients. Peripheral venous blood was taken,and Real-Time RT-PCR was used to detect the levels of IL-22 mRNA,IL-17 mRNA,and ELISA was used to detect the levels of IL-22,IL-17.Finally,relevant analysis was made between the test results and the condition of vitiligo patients. Results In the experimental group,vitiligo patients’ IL-22 mRNA IL-17 mRNA level in PBMC and IL-22,IL-17 were significantly higher than healthy controls,and the group of activity vitiligo patients was higher than vitiligo patients who were in stable stage. Ordinary vitiligo patients’ IL-22 mRNA,IL-17 mRNA levels in PBMC and IL-22,IL-17 protein levels in serum were significantly higher than segmental vitiligo patients. In vitiligo patients’ peripheral blood: IL-22 mRNA,IL-17 mRNA level and IL-22,IL-17 protein had significant correlation( r = 0. 753,P = 0. 000). Conclusions Th22 and Th17 cells may participate in the development of vitiligo.