蟾龙镇痛膏对骨转移性癌痛模型大鼠疼痛阈值及血清PGE2、TNF-α、IL-6、β-EP的影响

Effects of Chan Long Zhentong Paste on Pain Threshold and Serum Levels of PGE2,TNF-α,IL-6and β-EP in Bone Metastatic Cancer Pain Model Rats

目的探讨蟾龙镇痛膏对骨转移性癌痛大鼠模型的镇痛作用及其作用机制。方法将40只雌性SD大鼠随机分为空白组、模型组、蟾龙镇痛膏组(0.56 mg·kg-1)、扶他林组(0.08 mg·kg-1),每组10只。除空白组外,其余各组大鼠右后肢胫骨注射Walker256乳腺癌肿瘤细胞悬液,建立骨癌痛大鼠模型。造模后14 d开始外敷相应药物,每日1次,连续21 d。观察各组大鼠疼痛缩足反射潜伏期(PWL)和缩足反射阈值(PWT),通过HE染色、X线检查评价骨癌痛大鼠模型,并用酶联免疫吸附测定法(ELISA)检测血清前列腺素E2(PGE2)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、β内啡肽(β-EP)水平。结果模型组、蟾龙镇痛膏组、扶他林组PWL、PWT较空白组降低(P <0.05),HE染色观察可见肿瘤细胞,X线检查可见骨质破坏;药物干预后蟾龙镇痛膏组、扶他林组疼痛阈值较模型组上升(P <0.05);模型组血清PGE2、TNF-α、IL-6、β-EP含量较空白组升高(P <0.05),蟾龙镇痛膏组、扶他林组血清PGE2、TNF-α、IL-6含量较模型组降低(P <0.05);血清β-EP含量较模型组升高(P <0.05)。结论蟾龙镇痛膏对骨癌痛大鼠有镇痛作用,其机制可能与抑制炎性因子释放和提高内源性阿片肽有关。

Objective To explore the analgesic effect and possible mechanism of Chan Long Zhentong paste(CZP)in bone metastatic cancer pain model rats.Methods Forty female SD rats were randomly divided into control group,model group,CZP group and Voltaren group,with 10 rats in each group.Except the control group,rats in the other groups were injected with Walker256 breast cancer cell suspension in the right hind limb to establish the bone metastatic cancer pain model.Drugs treatments were started on the 14 th day after surgery,once a day for 21 days.The changes of paw withdraw latency(PWL)and paw withdraw threshold(PWT)in rats of each group were observed.The rat model of bone metastatic cancer pain was evaluated by hematoxylin-eosin staining(HE)and X-ray examination.The prostaglandin E2(PGE2),tumor necrosis factor-α(TNF-α),interleukin 6(IL-6),β-endorphin(β-EP)levels in rats’serum were detected by enzyme-linked immunosorbent assay(ELISA)method.Results The PWL and PWT of the rats in model group,CZP group and Voltaren group were lower than that in control group after model establishment(P<0.05).Tumor cells and bone destruction can be found in model group,CZP group and Voltaren group rats by HE staining and X-ray examination.The PWL and PWT of the rats in CZP group and Voltaren group were higher than those in model group rats after drug intervention(P<0.05).The serum levels of PGE2,TNF-α,IL-6 andβ-EP of rats in model group were higher than those in control group(P<0.05).The serum PGE2,TNF-α,IL-6 levels of the rats in CZP group and Voltaren group were lower than those in model group(P<0.05);the serumβ-EP levels of the rats in CZP group and Voltaren group were higher than those in model group(P<0.05).Conclusion The mechanism of CZP in bone metastatic cancer pain rats model may be related to the inhibition of inflammatory factors and the increase of endogenous opioid peptide.