精子DNA碎片指数对冷冻胚胎移植临床结局的影响

Effect of sperm DNA fragment index on clinical outcome of frozen embryo transfer

目的通过评估精子DNA碎片指数(DFI),探讨精子DFI对冷冻胚胎移植结局的影响。方法采用改良精子染色质扩散实验(SCD)对530例接受体外受精(IVF)和153卵细胞浆单精子注射(ICSI)的男方进行精子DFI检测,并根据精子DFI值将上述IVF患者分组(DFI≤30%,DFI>30%),根据单因素方差分析统计方法分析各组IVF受精率、卵裂率、优质胚胎率和冷冻胚胎移植的妊娠结局的差异。结果精子碎片指数DFI≤30%组在临床妊娠率,生化妊娠率和流产率与DFI>30%组相比没有显著变化。在精子DNA碎片指数≤30%的ICSI组,囊胚形成率显著高于DFI>30%组。结论精子染色质扩散试验(SCD)方法测定的精子DNA碎片指数与冷冻胚胎移植(FET)结局无关,但是与ICSI周期的囊胚形成率有关。

Objective:To investigate the effect of sperm DFI on the outcome of frozen embryo transfer by evaluating sperm DNA Fragment index(DFI).Methods:Sperm DFI were detected by modified sperm chromatin diffusion experiment(SCD)in 530 men with IVF(IVF)and 153 sperm injection(ICSI),and the patients were grouped according to sperm DFI value(DFI≤30%,DFI>30%),The differences of fertilization rate,cleavage rate,high quality embryo rate and pregnancy outcome of frozen embryo transfer were analyzed by single factor variance analysis and statistic method.Results:There was no significant change in the clinical pregnancy rate,biochemical pregnancy rate and abortion rate compared with sdf 30% group in the Sperm Fragment index sdf 30% group.The rate of blastocyst formation was significantly higher in the ICSI group with sperm DNA fragment index 30% than in DFI 30% group.Conclusion:The sperm DNA fragment index determined by the sperm chromatin diffusion test(SCD)was unrelated to the outcome of the frozen embryo Transfer(FET),but was related to the blastocyst formation rate in the ICSI cycle.