苇茎汤调控人肺腺癌A549细胞中EGFR、STAT3抗凋亡的机制研究

Mechanism of Weijing Decoction on anti-apoptosis by manipulating EGFR,STAT3 in human lung adenocarcinoma A549 cells

目的:研究苇茎汤对人肺腺癌A549细胞凋亡的影响。方法:将苇茎汤培养液浓度稀释为0.25、2.5、25g/L,与A549细胞共同培养,AO∕EB荧光染色观察细胞凋亡形态学改变;流式细胞术分析细胞周期和细胞凋亡率;Real-time PCR检测EGFR、STAT3基因的表达。结果:0.25~25g/L苇茎汤能诱导A549细胞发生凋亡形态学改变,且细胞凋亡率随苇茎汤浓度增加而升高;苇茎汤可将A549细胞周期阻滞于G0/G1期;Real-time PCR结果表明,苇茎汤可以下调A549细胞中EGFR、STAT3基因的表达。结论:苇茎汤可能通过调控EGFR、STAT3信号通路诱导人肺腺癌A549细胞发生早期凋亡。

Objective: In order to study the apoptosis inducing effect of Weijing decoction on human lung adenocarcinoma A549 cells.Methods: Weijing decoction were divided into 0.25,2.5,25 g/L to culture with A549 cells,AO ∕ EB fluorescent staining was used to observe A549 cells' morphological changes of apoptosis; Flow cytometry was used to analyse cells' cycle and apoptosis induction; Real-time PCR was used to determine expression of epidermal growth factor receptor(EGFR) and signal transducer and activator of transcription 3(STAT3) genes.Results: 0.25 ~ 25g/L Weijing decoction all can induce apoptosis and change appearance,and the apoptotic rate of cells was positively correlated with Weijing Decoction's dose; A549 Cells were inhibited in G0/G1 by Weijing Decoction; Real-time PCR assay showed that Weijing Decoction can inhibit the level of EGFR and STAT3 expression.Conclusion: Weijing decoction can induce early apoptosis of human lung adenocarcinoma A549 cells by regulating the signaling pathway of EGFR and STAT3.