番茄红素对缺铁性贫血大鼠铁利用和抗氧化作用研究

Effects of lycopene on iron utilization and antioxidative ability of iron deficiency anemia rats

目的:研究番茄红素(Lycopene,LP)对缺铁性贫血大鼠铁利用及机体抗氧化能力的影响。方法:采用初断乳Wistar大鼠低铁饲料和去离子水喂养4周的方法制备缺铁性贫血大鼠模型,选取60只随机分为5组:缺铁性贫血模型组、番茄红素5、10和20 mg/kg治疗组、硫酸亚铁(2 mg/kg)治疗组;另取同龄12只Wistar大鼠经正常饲料喂养作为正常对照组。灌胃给药,每天1次,疗程为4周。分别测定各组大鼠体重,肝脏和脾脏指数;测定血液指标:测定全血中血红蛋白含量,血清中血清铁、血清铁蛋白、总铁结合力、促红细胞生成素、转铁蛋白受体;测定各组大鼠肝脏和脾脏中铁含量;测定各组大鼠红细胞内游离原卟啉含量;测定血清中抗氧化性能监测指标:总抗氧化能力,超氧化物歧化酶、谷胱甘肽过氧化物酶活性及丙二醛含量。结果:与缺铁性贫血模型组相比,番茄红素5、10和20 mg/kg治疗组大鼠血中血红蛋白含量显著升高、超氧化物歧化酶活性显著升高、丙二醛含量显著降低;番茄红素10和20 mg/kg治疗组大鼠体质量显著升高、肝脏指数显著降低、脾脏指数显著降低,血中血清铁含量显著升高、总铁结合力显著降低,血清中血清铁蛋白显著升高、促红细胞生成素含量显著升高、转铁蛋白受体显著降低,肝脏中铁含量均显著升高、脾脏中铁含量均显著升高,红细胞内游离原卟啉含量显著降低,血清中谷胱甘肽过氧化物酶活性显著升高、总抗氧化能力水平显著升高。与硫酸亚铁对照组相比,番茄红素20 mg/kg治疗组大鼠体重,全血中促红细胞生成素,血清中血红蛋白、血清铁、总抗氧化能力,肝脏铁,超氧化物歧化酶、谷胱甘肽过氧化物酶活性显著升高;肝脏指数,血清中总铁结合力、转铁蛋白受体,红细胞内游离原卟啉含量均显著降低。结论:番茄红素能够有效增强缺铁性贫血大鼠铁利用能力和机体抗氧化能力,其作用优于硫酸亚铁。

Objective: To investigate the effects of Lycopene(LP) on iron utilization and antioxidative ability of iron deficiency anemia rats.Methods: Iron deficiency anemia ablactational Wistar rat models were made by feeding with iron deficiency forage and deionized water for four weeks,and selected 60 models were randomly devided into five groups: iron deficiency anemia model group,LP(5、10 and 20 mg/kg) treated groups and Ferrous Sulfate(2 mg/kg) treated group,and another normal 12 same-aged rats feed with common forage as normal control group.The drugs were given by intragastric administration for four weeks,once a day.The body weight,hepar index and spleen index were determined; the content of Hb,SI,SF,TIBC,EPO,s TfR in serum were determined; the content of iron in hepar and spleen were detected;the content of FEP were determined; and the antioxidant indexes in serum were also determined: the level of T-AOC,the activity of SOD and GSH-Px,the content of MDA.Results: Compared with the iron deficiency anemia model group,the content of Hb in blood of LP(5、10 and20 mg/kg) treated groups were significantly increased[(98±9),(107±12),(125±13) g/L vs(29.6±3.7) g/L; P<0.05,P<0.01],the content of MDA in serum were significantly decreased[(7.24±0.97),(6.69±0.88),(6.17±0.75) nmol/L vs(7.63±1.05)nmol/L; P<0.05,P<0.01],the activity of SOD in serum were significantly increased[(346±21),(365±22),(384±20) U/ml vs(317±18) U/ml; P<0.05,P<0.01].The body weight of LP(10 and 20 mg/kg) treated groups were significantly increased[(238 ±11),(244±13) g vs(227±12) g; P<0.05],hepar index were significantly decreased[(35.7±2.1),(35.2±2.2) ‰ vs(36.4±2.2)‰; P<0.05,P<0.01],spleen index were significantly decreased [(2.87±0.19),(2.83±0.19) ‰ vs(2.98±0.22) ‰; P<0.05,P<0.01],the content of SI in blood were significantly increased [(12.6±1.2),(14.8±1.5) mg/L vs(8.9±0.8) mg/L; P<0.01],TIBC were significantly decreased [(30.4±1.4),(28.7±1.5) mg/L vs(34.2±1.7) mg/L; P<0.05,P<0.01],the content of SF in serum were significantly increased[(46.7±4.4),(75.3±6.8) ng/ml vs(29.6±3.7) ng/ml; P<0.05,P<0.01],EPO were significantly increased [(11.3±0.9),(12.4±1.0) IU/L vs(9.3±0.8) IU/L; P<0.05,P<0.01],and s TfR significantly decreased [(5.48±0.37),(4.91±0.32) μg/ml vs(6.64±0.49) μg/ml; P<0.05,P<0.01],the content of FEP were significantly decreased [(462± 23),(414±20) μg/L vs(527±28) μg/L; P<0.05,P<0.01],the activity of GSH-Px in serum were significantly increased[(856 ±53),(884±57) U/ml vs(814±48) U/ml; P<0.05],the level of T-AOC were significantly increased[(12.5±2.0),(13.2±2.2) U /ml vs(11.2±1.9) U/ml; P<0.05].Compared with Ferrous Sulfate treated group,the body weight and the level of T-AOC,the content of EPO in serum,the activity of SOD and GSH-Px of LP 20 mg/kg treated groups were significantly increased(P<0.05,P<0.01); and the hepar index,the content of TIBC,s TfR,FEP in serum were significantly decreased(P<0.05).Conclusion: Lycopene could effectively improve the iron utilization and antioxidative ability of iron deficiency anemia rats.Compared with Ferrous Sulfate,the effects of Lycopene on iron utilization and antioxidative ability were better.