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紫萁对内毒素诱导的急性肺损伤保护作用机制研究

Ziqi water extract attenuates acute lung injury induced by lipopolysaccharide in mice
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摘要 目的:探讨紫萁水提物对内毒素(lipopolysaccharide,LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用。方法:于小鼠腹腔注射LPS(5 mg/kg)复制ALI动物模型。将小鼠随机分为空白组对照组、LPS组、地塞米松组(2 mg/kg)、紫萁水提物组(4g/kg、8g/kg)。观察各组肺组织病理学改变,测量肺湿/干重比,支气管肺泡灌洗液中性粒细胞百分比、超氧化歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、前列素E2(PGE2)含量及肺组织中环氧酶-2(COX-2)转录因子(NF-кBP65)蛋白表达。结果:紫萁水提物组(4g/kg、8g/kg)可有效减轻LPS所致肺组织病理学变化,能降低肺泡灌洗液中炎症细胞的数目、MDA、NO、IL-6、TNF-α、PGE2,增加肺泡灌洗液中SOD;著降低肺湿/干重比重,减低COX-2与NF-кBP65蛋白水平。结论:紫萁水提物组(4g/kg、8g/kg)可减轻LPS所致急性肺组织损伤,对LPS诱导的急性肺损伤有保护作用。 Objective: To determine the protective effect of Ziqi water extract on acute lung injury induced by lipopolysaccharide(LPS).Methods:The BABL/C mice were randomly allocated into control,LPS,dexamethasone(2mg/kg),Ziqi water extract(4g/kg),and Ziqi water extract(8g/kg).The bronchoalveolar lavage fluid(BALF) samples were analyzed for the concentration of numbers of neutrophilic,SOD,MDA,NO,prostaglandin E2(PGE2),interleukin-6(IL-6) and tumor necrosis factor alpha(TNF-α),and the lung samples were taken for histologic evaluation and for determination of wet-to-dry(W/D) lung weight,The protein levels of cyclooxygenase-2(COX-2) were detected by Western blot analysis.Results: Pretreatment with Ziqi water extract prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight/dry weight ratio,in total leukocyte number and neutrophil percent in the BALF,and in PGE2 of lung in dose-dependent manners,and in IL-6,the tumor neurosis factor-α(TNF-α) in the BALF and COX-2 protein in lung.These results showed that anti-inflammatory effects of Ziqi water extract against the LPS-induced ALI.
出处 《中药药理与临床》 CAS CSCD 北大核心 2015年第2期43-45,共3页 Pharmacology and Clinics of Chinese Materia Medica
基金 吉林省科学技术厅科技引导计划项目(200905110)
关键词 紫萁 急性肺损伤 COX-2 Ziqi(紫萁) LPS-induced acute lung injury COX-2
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参考文献5

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