肠胃清抗结肠癌增殖作用研究

Research of Changweiqing anti-proliferative effect on colon cancer

目的:探讨肠胃清对结肠癌细胞增殖、凋亡的作用。方法:采用显微镜、电镜、MTT、流式细胞仪检测肠胃清对结肠癌LoVo细胞形态、结构、增殖、周期、凋亡的影响。结果:肠胃清改变结肠癌LoVo细胞形态,不影响其结构,肠胃清0.98g/L组、肠胃清1.97 g/L组、肠胃清3.93 g/L组、奥沙利铂9.23×10-3g/L组、肠胃清1.97 g/L+奥沙利铂9.23×10-3g/L组对LoVo细胞的抑制率依次为:7.91±0.58、30.01±0.62、62.36±1.12、24.8±1.04、31.42±0.68,组间差异显著;空白对照组、肠胃清0.98g/L组、肠胃清1.97 g/L组、肠胃清3.93 g/L组、奥沙利铂9.23×10-3g/L组、肠胃清1.97 g/L+奥沙利铂9.23×10-3g/L组LoVo细胞G1期、G2期、S期百分比分别依次为:65.00±2.64、62.41±4.57、59.18±1.56、53.71±3.73、45.19±1.00、44.79±2.23;22.86±0.04、24.60±5.48、27.94±2.71、34.99±3.27、44.20±0.43、44.70±0.95;14.38±1.22、14.29±1.57、13.81±1.42、11.63±3.31、11.40±0.88、9.72±0.10,组内无显著差异。对LoVo细胞的凋亡率依次为9.06±1.22、10.47±2.12、11.19±1.41、12.68±0.55、16.24±1.25、20.68±1.57。结论:肠胃清不影响结肠癌LoVo细胞的结构,抑制其增殖,改变其周期,促进其凋亡,与化疗药有协同作用。

Objective: To discuss the effect of Changweiqing on apoptosis and proliferation of colon cancer.Methods: By microscopy,electron microscopy,MTT,flow cytometry the affection of LoVo cell of colon cancer morphology,structure,proliferation,cell cycle,and apoptosis was observed with Changweiqing.Results: With Changweiqing being done,morphology of colon cancer LoVo cell was changed,but its structure did not been affected.The inhibition rate on LoVo cells with Changweiqing 0.98 g/L,1.97 g/L,3.93 g/L group,Oxaliplatin 9.23 × 10-3g/L group,Changweiqing1.97 g/L + Oxaliplatin 9.23 × 10-3g/L group were as follows: 7.91±0.58,30.01±0.62,62.36±1.12,24.8±1.04,31.42±0.68,and it was significant difference between groups.By the control group,Changweiqing 0.98 g/L,1.97 g/L,3.93 g/L group,Oxaliplatin 9.23 × 10-3g/L group,Changweiqing1.97 g/L + Oxaliplatin 9.23 × 10-3g/L group,the percentages of LoVo cells in G1 phase,G2 phase,S phase were respective successively: 65.00±2.64,62.41±4.57,59.18±1.56,53.71±3.73,45.19±1.00,44.79±2.23; 22.86± 0.04,24.60±5.48,27.94±2.71,34.99±3.27,44.20±0.43,44.70±0.9; 514.38±1.22,14.29±1.57,13.81±1.42,11.63±3.31,11.40±0.88,9.72±0.10,it was not significant difference within the groups.Furthermore,the apoptosis rates of LoVo cells with groups were in proper order: 9.06±1.22,10.47±2.12,11.19±1.41,12.68±0.55,16.24±1.25,20.68±1.57.Conclusion: Changweiqing did not affect structure of LoVo cells,but changed cycle of LoVo cells,induced their apoptosis,and inhibitted their proliferation,simultaneously,there was a synergistic effect with chemotherapy drugs.