人参炙甘草附子配伍对小鼠急性毒性的影响

Combination of ginseng, roasted liquorice and radix aconiti chamichaeli and their effect on the mice acute toxicity

目的:探讨附子与人参和炙甘草配伍对小鼠急性毒性的影响,旨在从毒性角度考察人参附子配伍、炙甘草附子配伍、人参炙甘草附子配伍是否减毒,为临床安全合理用药提供依据。方法:分别制备附子水提、人参附子水提、炙甘草附子水提和人参炙甘草附子水提药物,采用经典的急性毒性实验方法测定其半数致死量LD50。结果:附子水提组分LD50为22.862 g/kg,人参附子水提组分LD50为31.346 g/kg,炙甘草附子水提组分LD50为29.804 g/kg,人参炙甘草附子水提组分LD50为29.962 g/kg。通过观察小鼠自主活动,死亡情况、摄食量、体质量和心、肝、脾、肺、肾各脏器指数的变化,可知人参附子配伍、炙甘草附子配伍和人参炙甘草附子配伍具有一定的减毒作用。总结:人参、炙甘草对附子具有一定的减毒作用,其减毒强度为:人参附子配伍>人参炙甘草附子配伍>炙甘草附子配伍。

Objective:To explore the effects on mice acute toxicity of combination,to investigate the attenuate effect of different combination of ginseng,roasted liquorice and radix aconiti chamichaeli,which provides a basis for clinical safe and rational application.Methods:radix aconiti chamichaeli water extract,ginseng combined radix aconiti chamichaeli water extract,roasted liquorice combined radix aconiti chamichaeli water extract and ginseng combined radix aconiti chamichaeli,roasted liquorice water extract were separately prepared.The lethal dose LD50 was measured by acute toxicity method.Results:LD50 of radix aconiti chamichaeli water extract was 22.862 g/kg,LD50 of ginseng combined with radix aconiti chamichaeli water extract was 31.346 g/kg,LD50 of roasted liquorice combined with radix aconiti chamichaeli water extract was 29.804 g/kg,and LD50 of ginseng combined with radix aconiti chamichaeli,roasted liquorice water extract was 29.962 g/kg.The spontaneous activity and behavioral changes of mice were observed,the death,food intake and index of body weight,heart,liver,spleen,lung and kidney were recorded to study the attenuating effects of different combination.Conclusion:Ginseng and roasted liquorice have certain attenuating effects on radix aconiti chamichaeli.Their attenuating effects were shown in order:ginseng combined with radix aconiti chamichaeli>ginseng combined with roasted liquorice and radix aconiti chamichaeli>roasted liquorice combined with radix aconiti chamichaeli.