在线固相萃取-HPLC测定三七消痔栓中的三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量测定

Determination of Notoginsenoside R_1 Ginsenoside Rg_1,and Ginsenoside Rb_1,Contents in Sanqixiaozhishuan by Online Solid Phase Extraction-HPLC

目的:建立三七消痔栓中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1含量测定的方法。方法:运用在线固相萃取-HPLC测定三七消痔栓中的三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1的含量。结果:含量测定结果准确,方法简便,专属性强:三七皂苷R_1(C_(47)H_(80)O_(18))、人参皂苷Rg_1(C_(42)H_(72)O_(14))和人参皂苷Rb_1(C_(54)H_(92)O_(23))的平均回收率分别为97.21%(RSD=1.75%,n=6),99.26%(RSD=1.80%,n=6),100.48%(RSD=1.37%,n=6)。三七皂苷R_1、参皂苷Rg_1和人参皂苷Rb_1分别在2.794~27.94μg/mL,8.125~81.25μg/mL和8.088~80.88μg/mL范围内呈良好的线性关系。结论:本方法可有效控制三七消痔栓中三七的质量。

Objective:To establish a method for determination of the contents of notoginsenoside R_1,ginsenoside Rg,and ginsenoside Rb,in sanqixiaozhishuan.Methods:The contents of notoginsenoside R_1,ginsenoside Rg,and ginsenoside Rb,in sanqixiaozhishuan was determined by online solid phase extraction-HPLC.Results:The developed method was accurate,simple and specific.The average recovery rates of notoginsenoside R_1,ginsenoside Rg,and ginsenoside Rb,in sanqixiaozhishuan were 97.21%(RSD=1.75%,n = 6),99.26%(RSD= 1.80%,n=6) and 100.48%(RSD= 1.37%,n=6),while the linear ranges were 2.794~27.94,8.125~81.25 and 8.088~80.88 μg/mL,respectively.Conclusions:The method may be used for effective control of the quality of pseudo-ginseng in sanqixiaozhishuan.