1,25-(OH)_2-D_3对脑梗死大鼠神经功能、脑组织内细胞凋亡及Wnt/β-catenin通路的调节作用

Regulation Effect of 1,25-(OH)_2-D_3 on Neurological Function, Apoptosis in Brain Tissues and Wnt/β-catenin Pathway in Rats with Cerebral Infarction

目的:研究1,25-(OH)_2-D_3对脑梗死大鼠神经功能、脑组织内细胞凋亡及Wnt/β-catenin通路的调节作用。方法 :选择成年雄性SD大鼠,随机分为假手术组、脑梗死组、干预组,脑梗死组和干预组采用线栓法建立脑梗死模型,干预组给予1,25-(OH)_2-D_3干预。评价三组大鼠的神经功能、脑梗死面积,采用TUNEL试剂盒检测细胞凋亡,采用Western-blot检测凋亡基因及Wnt/β-catenin通路基因的表达。结果:脑梗死组、干预组大鼠的神经功能评分、脑组织TUNEL阳性率及脑组织中Bax、Caspase-3、GSK-3β的表达量明显高于假手术组,脑组织中Bcl-2、β-catenin的表达量均明显低于假手术组,差异均有统计学意义(P <0.05);干预组大鼠的神经功能评分、脑梗死体积、脑组织TUNEL阳性率及脑组织中Bax、Caspase-3、GSK-3β的表达量均明显低于脑梗死组,脑组织中Bcl-2、β-catenin的表达量均明显高于脑梗死组,差异均有统计学意义(P <0.05)。结论:1,25-(OH)_2-D_3干预脑梗死大鼠能够改善神经功能、减轻脑组织内细胞凋亡,分子机制可能为激活Wnt/β-catenin通路。

Objective:To study the regulation effect of 1,25-(OH)2-D3 on neurological function,apoptosis and Wnt/β-catenin pathway in brain tissues in the rats with cerebral infarction.Methods:Adult male Sprague-Dawley rats were selected and randomly divided into a sham operation group,a cerebral infarction group and an intervention group.Cerebral infarction models were established by thread embolism in the cerebral infarction group and the intervention group.The rats in the intervention group were given 1,25-(OH)2-D3 intervention.The neurological function and cerebral infarction area in the three groups were evaluated.The apoptosis was detected by a TUNEL kit.The expressions of apoptotic genes and Wnt/β-catenin pathway genes were detected by Westernblot.Results:The neurological function scores,the TUNEL positive rate of brain tissues and the expressions of Bax,Caspase-3 and GSK-3βin brain tissues in the cerebral infarction group and the intervention group were significantly higher than those in the sham operation group,and the expressions of Bcl-2 andβ-catenin were significantly lower than those in the sham operation group,with statistically significant differences(P<0.05).The neurological function scores,cerebral infarction volume,the TUNEL positive rate of brain tissues and the expressions of Bax,Caspase-3 and GSK-3βin brain tissues in the intervention group were significantly lower than those in the cerebral infarction group,and the expressions of Bcl-2 andβ-catenin in brain tissues were significantly higher than those in the cerebral infarction group,with statistically significant differences(P<0.05).Conclusion:1,25-(OH)2-D3 intervention on rats with cerebral infarction can improve neurological function and reduce apoptosis in brain tissues,and the molecular mechanism may be associated with activate Wnt/β-catenin pathway.